Leukemia Inhibitory Factor Enhanced the Developmental and Implantation Compatibility of Mouse Embryos in Co-culture with Human Endometrial Epithelial Cells  

作　　者：Ali Hosseini Bahar Movaghar Showra Amani Abkenari Hassan Nazari Mehrdad Bakhtiyari 

机构地区：[1]Department of Anatomy,Faculty of Medicine,Iran University of Medical Sciences,Tehran,Iran [2]Cellular and Molecular Research Center,Iran University of Medical Sciences,Tehran,Iran [3]Department of Embryology,Reproductive Biomedicine Research Center,Royan Institute for Reproductive Biomedicine,ACECR,Tehran,Iran [4]Department of Anatomy,School of Medicine,Tehran University of Medical Sciences,Tehran,Iran [5]Research Institute of Animal Embryo Technology,Shahrekord University,Shahrekord,Iran

出　　处：《Reproductive and Developmental Medicine》2021年第4期199-205,共7页生殖与发育医学（英文版）

摘　　要：Objective:Among the variousin vitro embryo culture systems,co-culture has demonstrated remarkable effects in pre-implantation embryo development owing to the production of embryo-nourishing factors.Nevertheless,little is known about the secretion of these factors.Therefore,in this study,the effect of leukemia inhibitory factor(LIF),one of the most important nourishing factors in the early development of mouse embryos,in human endometrial epithelial cells(hEECs)was evaluated.Methods:Two-cell stage embryos were collected from the oviducts of hyper-stimulated and mated mice and cultivated in a co-culture with an hEEC monolayer with or without LIF.The quality and developmental and attachment potential rates of cultured embryos were evaluated by determining the levels of octamer-binding transcription factor 4(Oct4)and caudal type homeobox 2(Cdx2)transcripts.Results:LIF significantly increased the developmental rate(82.67%vs.61.04%,respectively)and attachment rate(64%vs.45.45%,respectively)of mouse embryos co-cultured with hEECs compared to those in untreated embryos.The expression levels ofOct4 andCdx2 in blastocysts cultured in the presence of LIF were higher than those in blastocysts cultured without LIF.Conclusions:Despite the secretion of LIF by hEECs during co-culture with embryos,the amount of this factor was insufficient,and its addition to the culture media could increase the developmental potential of embryos.

关 键 词：Caudal Type Homeobox 2 Embryo Development Leukemia Inhibitory Factor Octamer-Binding Transcription Factor 4 

分 类 号：R714.1[医药卫生—妇产科学]