Combined enzymatic hydrolysis and herbal extracts fortification to boost in vitro antioxidant activity of edible bird's nest solution  

作　　者：Pei Ling Tang Hooi Shin Goh Swee Seng Sia 

机构地区：[1]Department of Bioscience,Faculty of Applied Sciences,Tunku Abdul Rahman University College,Setapak,53300 Kuala Lumpur,Malaysia [2]Nestlin Malaysia Sdn.Bhd.,3A,Jalan Payamas,Taman Industri Payamas,Tangkak 84900,Johor,Malaysia

出　　处：《Chinese Herbal Medicines》2021年第4期549-555,共7页中草药（英文版）

基　　金：supported by the teaching activity fund of Department of Bioscience,Faculty of Applied Sciences,Tunku Abdul Rahman University College。

摘　　要：Objective:Edible bird’s nest(EBN)is a popular traditional tonic food in Chinese population for centuries.Malaysia is one of the main EBN suppliers in the world.This study aims to explore the best strategy to boost the antioxidant potential of EBN solution.Methods:In this study,the raw EBN(4%,mass to volume ratio)was initially enzymatic hydrolyzed using papain enzyme to produce EBN hydrolysate(EBNH),then spray-dried into powdered form.Next,4%(mass to volume ratio)of EBNH powder was dissolved in ginger extract(GE),mulberry leaf extract(MLE)and cinnamon twig extract(CTE)to detect the changes of antioxidant activities,respectively.Results:Results obtained suggest that enzymatic hydrolysis significantly reduced the viscosity of 4%EBN solution from(68.12±0.69)m Pa·s to(7.84±0.31)m Pa·s.Besides,the total phenolic content(TPC),total flavonoid content(TFC),total soluble protein,DPPH scavenging activity and ferric reducing antioxidant power(FRAP)were substantially increased following EBN hydrolysis using papain enzyme.In addition,fortification with GE,MLE and CTE had further improved the TPC,TFC,DPPH scavenging activity and FRAP of the EBNH solution.Among the samples,MLE-EBNH solution showed the most superior antioxidant potential at(86.39±1.66)%of DPPH scavenging activity and(19.79±2.96)mmol/L FeSO;of FRAP.Conclusion:This study proved that combined enzymatic hydrolysis and MLE fortification is the best strategy to produce EBN product with prominent in vitro antioxidant potential.This preliminary study provides new insight into the compatibility of EBN with different herbal extracts for future health food production.

关 键 词：antioxidant cinnamon twig edible bird’s nest enzymatic hydrolysis GINGER mulberry leaves 

分 类 号：TS218[轻工技术与工程—粮食、油脂及植物蛋白工程]