基于基因芯片的儿童皮肌炎生物信息学分析  

Bioinformatics analysis of juvenile dermatomyositis by microarray

作　　者：岑昕陶 魏姗姗 马思宁 闫璐[1] 孙乐栋[1,2] CEN Xin-tao;WEI Shan-shan;MASi-ning(Department of Dermatology,Zhujiang Hospital,the Southern Medical University,Guangzhou 510282,China)

机构地区：[1]南方医科大学珠江医院皮肤科,广州510282 [2]南方医科大学第五附属医院,广州510282

出　　处：《实用皮肤病学杂志》2021年第6期326-330,共5页Journal of Practical Dermatology

摘　　要：目的 通过生物信息学方法,探究与儿童皮肌炎相关的差异基因,进一步阐明儿童皮肌炎的发病机制。方法 选取基因表达汇编数据库的基因表达芯片数据(GSE11083),利用R语言筛选差异基因。使用Metascape在线工具进行GO功能和KEGG通路富集分析,同时利用String数据库和Cytoscape软件进行蛋白相互作用网络分析,并筛选儿童皮肌炎发病的关键基因。结果 筛选出差异基因共179个,其中表达上调3个,表达下调176个。GO分析显示差异基因主要富集在中性粒细胞活化、自噬和抗微生物体液反应等生物学过程。KEGG分析显示差异基因主要富集在Fc受体介导的吞噬作用、T淋巴细胞受体等信号通路。筛选获得的5个关键基因为PTPRC、LAMTOR1、CLEC12A、ITGAV和RAB5C。结论 通过对儿童皮肌炎基因芯片的生物信息学分析,PTPRC、LAMTOR1与CLEC12A等基因可能参与儿童皮肌炎的发病机制。Objective To screen differentially expressed genes(DEGs) and to explore the pathogenesis of juvenile dermatomyositis by bioinformatic analysis. Methods The GSE11083 dataset was downloaded from the Gene Expression Omnibus(GEO) database, and the differentially expressed genes analysis was performed by using R software. Metascape database was used to perform GO function analysis and KEGG pathway enrichment analysis. The String database and Cytoscape software were used to construct a protein-protein interaction(PPI) network and identify the key genes involved in juvenile dermatomyositis. Results A total of 179 differentially expressed genes were screened out, of which 176 genes were down-regulated and 3 genes were up-regulated. The differentially expressed genes of juvenile dermatomyositis were mainly concentrated in the biological processes such as neutrophil activation, autophagy and antimicrobial humoral response. KEGG pathway enrichment analysis showed that the DEGs were mainly enriched in endocytosis, Fc gamma R-mediated phagocytosis, and T cell receptor signaling pathway. The 5 key genes identified were PTPRC, LAMTOR1, CLEC12A, ITGAV, and RAB5C.Conclusion The results of our bioinformatics analysis of gene microarray in juvenile dermatomyositis suggested that the genes such as PTPRC, LAMTOR1 and CLEC12A may be involved in the pathogenesis of juvenile dermatomyositis.

关 键 词：皮肌炎 儿童 生物信息学 GEO数据库 差异基因 

分 类 号：R593.26[医药卫生—内科学]