当归多糖对妊娠期易栓症大鼠的抗炎作用及对凝血功能的影响  

Anti-inflammation Effect of Angelica Polysaccharide in Rats with Thrombophilia During Pregnancy and Impact on Coagulation Function

作　　者：麻卲㛃 李晓茹[1] 吕志华[1] 吴纪珍[2] MA Shao-Jie;LI Xiao-Ru;LYU Zhi-Hua;WU Ji-Zhen(Dept.of Gynecology,Zhengzhou First People’s Hospital,Zhengzhou 450000 Henan,China;Dept.of Critical Care Medicine,Henan Provincial People’s Hospital,Zhengzhou 450000 Henan,China)

机构地区：[1]郑州市第一人民医院妇科,河南郑州450000 [2]河南省人民医院危重症医学科,河南郑州450000

出　　处：《广州中医药大学学报》2022年第3期612-617,共6页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：河南省科技研发专项项目(编号:162102310032)。

摘　　要：【目的】探讨当归多糖对妊娠期易栓症大鼠的抗炎作用及对凝血功能的影响。【方法】随机留取15只雌性大鼠为正常组,其余70只发情期雌性大鼠妊娠后尾静脉注射10 mg/mL脂多糖(LPS)建立易栓症模型。将建模成功的49只雌性大鼠随机分为模型组,当归多糖低、中、高剂量组。当归多糖低、中、高剂量组分别灌胃50、100、200 mg/kg当归多糖,模型组和正常组灌胃等体积生理盐水,每日1次,连续7 d。末次给药30 min后,分别用断尾法和玻片法测定出血和凝血时间;干预结束后,酶联免疫吸附分析(ELISA)测定大鼠血清白细胞介素6(IL-6)、肿瘤坏死因子α(TNF-α)、基质细胞衍生因子1(SDF-1)水平和血浆组织因子(TF)、抗凝血酶Ⅲ(AT-Ⅲ)、D-二聚体(D-D)、纤维蛋白原(FIB)水平,苏木素-伊红(HE)染色观察大鼠含血栓静脉管壁病理学变化。【结果】与正常组比较,模型组出血时间增加,凝血时间减少,血清IL-6、TNF-α、SDF-1,血浆TF、D-D、FIB水平升高,AT-Ⅲ水平降低(P<0.05)。与模型组比较,当归多糖低、中、高剂量组出血时间减少,凝血时间增加,血清IL-6、TNF-α、SDF-1及血浆TF、D-D、FIB水平降低,AT-Ⅲ水平升高,且呈剂量依赖性(P<0.05)。HE染色结果显示:正常组血管内壁正常,无炎性细胞浸润;模型组血管内壁增生,大量钙盐及含铁血黄素析出,新生毛细血管数量增多,血栓形成;当归多糖各组血栓明显减少,其中以高剂量组改善最明显。【结论】当归多糖对妊娠期易栓症大鼠具有抗炎、抗凝血作用。Objective To explore the anti-inflammatory effect of Angelica polysaccharide(AP)in rats with thrombophilia during pregnancy and effect on coagulation function.Methods Fifteen female rats were randomly set aside as the normal group,and the remaining 70 female rats in oestrus were injected with 10 mg/mL lipopolysaccharide(LPS)into the tail vein after pregnancy to establish a model of thrombophilia.The 49 female rats successfully modeled were randomly divided into the model group and the AP low-,medium-and high-dose groups.The AP low-,medium-and high-dose groups were gavaged with 50,100 and 200 mg/kg of AP respectively,while the model group and normal group were gavaged with an equal volume of normal saline,once a day,for 7 consecutive days.Thirty minutes after the last intervention,the bleeding and coagulation time of each group was measured by tail amputation method and slide method respectively.The levels of serum interleukin 6(IL-6),tumor necrosis factorα(TNF-α),stromal cell derived factor 1(SDF-1),plasma tissue factor(TF),antithrombinⅢ(AT-Ⅲ),D-dimer(D-D)and fibrinogen(FIB)were determined by enzyme-linked immunosorbent assay(ELISA)after the intervention.The pathological changes of vein wall with thrombus in rats was observed by hematoxylin-eosin(HE)staining.Results Compared with the normal group,the model group showed increased bleeding time,decreased coagulation time,increased level of serum IL-6,TNF-α,SDF-1,plasma TF,D-D and FIB,and decreased level of AT-Ⅲ(P<0.05).Compared with the model group,the low-,medium-and high-dose groups of AP showed decreased bleeding time,increased coagulation time,decreased levels of serum IL-6,TNF-α,SDF-1,plasma TF,D-D,FIB,and increased level of AT-Ⅲin a dosedependent manner(P<0.05).HE staining results showed that the inner wall of blood vessels in the normal group was normal without inflammatory cell infiltration;the inner wall of blood vessels in the model group was hyperplastic,a large amount of calcium salts and iron-containing haematoxylin were precipitated,the nu

关 键 词：当归多糖 易栓症 妊娠 炎症 凝血 大鼠 

分 类 号：R285.5[医药卫生—中药学]