振腹环揉法对PCPA失眠大鼠脑、肠组织中SP及GAL蛋白表达的影响  

Effect of the vibro-annular abdominal massage on the protein expression levels of SP and GAL in the brain and gut tissues of PCPA insomnia rats

作　　者：郅晓宇 刘鹏[2] 董娜 张野[1] 张红石[1] ZHI Xiaoyu;LIU Peng;DONG Na;ZHANG Ye;ZHANG Hongshi(Changchun University of Chinese Medicine,Changchun 130117,China;Affiliated Hospital of Changchun University of Chinese Medicine,Changchun 130021,China)

机构地区：[1]长春中医药大学,长春130117 [2]长春中医药大学附属医院,长春130021

出　　处：《长春中医药大学学报》2022年第4期394-397,共4页Journal of Changchun University of Chinese Medicine

基　　金：国家自然基金面上项目(82074569);吉林省教育厅“十三五”科学技术项目(JJKH20200880KJ);吉林省卫生健康科技能力提升项目(2021JC068)。

摘　　要：目的探讨振腹环揉法对PCPA失眠大鼠脑肠组织中SP及GAL蛋白表达的影响,验证腹部推拿治疗失眠存在脑肠互动现象。方法64只雄性Wistar大鼠随机分为空白组、模型组、推拿组、药物组。除空白组外均采用PCPA法建立失眠大鼠模型,造模成功后对推拿组进行振腹环揉法腹部推拿干预,药物组按人鼠比例进行人参归脾丸灌胃。分别于第5天、第10天取下丘脑及小肠冷冻匀浆,通过Western blot检测P物质(SP)、甘丙肽(GAL)在下丘脑及小肠(中段)中的蛋白表达。结果模型组与空白组比较,下丘脑及小肠中SP蛋白表达量均降低,下丘脑中GAL蛋白表达量降低,小肠中GAL蛋白表达量升高;推拿组与模型组比较,下丘脑及小肠中SP蛋白表达量均升高,下丘脑中GAL蛋白表达量升高,小肠中GAL蛋白表达量降低,以上结果差异均具有统计学意义(P<0.05)。结论振腹环揉法通过调节PCPA失眠大鼠SP及GAL的表达,改善失眠大鼠睡眠状态及肠道蠕动,这种现象验证了腹部推拿治疗失眠存在脑肠互动现象。Objective To investigate the effect of the vibro-annular abdominal massage on the protein expression levels of substance P(SP)and galanin(GAL)in the brain and gut tissues of PCPA insomnia rats,and to verify the brain-gut interaction phenomenon in the treatment of insomnia by the abdominal massage manipulation.Methods A total of sixty-four male Wistar rats were randomly divided into a blank group,a model group,a massage group and a drug group.Except the blank group,an insomnia rat model was established by the PCPA method.After that,the massage group was intervened by a vibro-annular abdominal massage manipulation,and the drug group was gavaged with Renshen Guipi Pills according to the human-rat ratio.The frozen homogenates in the hypothalamus and small intestine were collected on the 5th and 10th day respectively.The protein expression levels of SP and GAL in the hypothalamus and small intestine(middle section)were detected by Western blot.Results Compared with the blank group,the protein expression levels of SP in the hypothalamus and small intestine of the model group were both decreased,and the protein expression of GAL in the hypothalamus was decreased,while the protein expression of GAL in the small intestine was increased.Compared with the model group,the protein expression levels of SP in the hypothalamus and small intestine in the massage group were increased,and the protein expression level of GAL in the hypothalamus was increased,while the protein expression level of GAL in the small intestine was decreased.All the differences in the above results were statistically significant(P<0.05).Conclusion The vibro-annular abdominal massage manipulation can improve the sleep state and intestinal peristalsis by regulating the expression of SP and GAL in PCPA insomnia rats,which verifies the brain-gut interaction phenomenon in the treatment of insomnia by the abdominal massage manipulation.

关 键 词：振腹环揉法 失眠 P物质 甘丙肽 

分 类 号：R244.1[医药卫生—针灸推拿学]