穿龙薯蓣皂苷元对CIA小鼠CD4^(+)CD25^(-)T分化为Treg细胞的诱导作用  

Differentiation of CD4^(+)CD25^(-)T Cells to Regulatory T Cells induced by Diosgenin in CIA Mice

作　　者：梁秀军[1] 于永洲 夏东帅 高亚贤[1] 孙同友[3] LIANG Xiu-jun;YU Yong-zhou;XIA Dong-shuai;GAO Ya-xian;SUN Tong-you(Chengde Medical University,Chengde,067000,China;Hebei Key Laboratory of Nerve Injury and Repair,Chengde 067000,China;Chengde Central Hospital,Chengde,067000,China)

机构地区：[1]承德医学院,承德067000 [2]河北省神经损伤与修复重点实验室,承德067000 [3]承德市中心医院,承德067000

出　　处：《神经药理学报》2021年第2期5-10,共6页Acta Neuropharmacologica

基　　金：河北省高等学校科学技术研究项目(No.QN2016031);河北省中医药管理局科研计划项目(No.2015155);河北省科技厅中央引导地方科技发展基金项目(No.216Z2501G)。

摘　　要：目的:探讨穿龙薯蓣皂苷元诱导胶原性关节炎(collagen induced arthritis,CIA)小鼠CD4^(+)CD25^(-)T细胞向调节性T细胞(regulatory T cells,Treg)转化的影响。方法:采用免疫磁性细胞分离法(magnetic activated cell sorting,MACS)分离CIA小鼠的CD4^(+)CD25^(-)T细胞。通过不同浓度(25、12.5、6.25μmol·L^(-1))的穿龙薯蓣皂苷元诱导CD4^(+)CD25^(-)T细胞向Treg细胞分化,用FCM检测Treg细胞比率,Real-Time PCR法检测Foxp3 mRNA的水平,ELISA法检测培养上清白细胞介素10(interleukin 10,IL-10)的含量。结果:免疫磁性细胞分离法分选出的CD4^(+)CD25^(-)T细胞纯度达93.89%。与对照组相比,穿龙薯蓣皂苷元给药组Treg细胞比率明显上调(P<0.05),Foxp3 mRNA的表达水平和培养上清中IL-10的水平均明显增加(P<0.05)。结论:穿龙薯蓣皂苷元可以诱导CIA小鼠CD4^(+)CD25^(-)T细胞向Treg细胞分化,并促进Foxp3的表达。Objective:To investigate the roles of diosgenin in the differentiation of regulatory T cells from CD4^(+)CD25^(-)T cells in collagen induced arthritis mice.Methods:CD4^(+)CD25^(-)T cells of CIA mice isolated by immunomagnetic beads on magnetic activated cell sorting device were co-cultured with different concentrations of diosgenin(25、12.5、6.25μmol·L^(-1))for 48 hours.The ratio of Treg cells was tested by flow cytometry.The level of Foxp3 mRNA was measured by Real-Time PCR.The level of serum IL-10 was detected by ELISA.Results:This study separated CD4^(+)CD25^(-)T cells of 93.89%purity.The ratio of Treg cells,the level of IL-10 and Foxp3 mRNA were increased significantly in diosgenin groups compared with those in control group(P<0.05).Conclusion:Diosgenin may induce the differentiation of CD4^(+)CD25^(-)T cells to regulatory T cells in CIA mice and up-regulate the expression of Foxp3.

关 键 词：CIA模型 薯蓣皂苷元 调节性T细胞 

分 类 号：R392.5[医药卫生—免疫学]