血管生成素在凶险性前置胎盘孕妇中表达的临床意义  

Clinical significance of angiogenin expression in patients with pernicious placenta previa

作　　者：武建利[1] 李玲[1] 朱启英[1] WU Jianli;LI ling;ZHU Qiying(Department of Obstetrics,First Affiliated Hospital of Xinjiang Medical University,Urumqi Xinjiang 830054,China)

机构地区：[1]新疆医科大学第一附属医院母胎医学中心产科,新疆乌鲁木齐830054

出　　处：《中国优生与遗传杂志》2021年第11期1549-1552,共4页Chinese Journal of Birth Health & Heredity

基　　金：新疆维吾尔自治区自然科学基金项目(2017D01C317)。

摘　　要：目的通过检测血管生成素(ANG)在凶险性前置胎盘孕妇及正常妊娠孕妇外周血和胎盘组织中表达的差异性,探讨ANG在凶险性前置胎盘中表达的意义。方法采用ELISA、RT-PCR及免疫组化法检测凶险性前置胎盘孕妇20例及正常妊娠孕妇15例外周血和胎盘组织中ANG的表达以及定位情况。结果凶险性前置胎盘孕妇血清中ANG浓度明显高于正常妊娠孕妇(P<0.05);凶险性前置胎盘孕妇胎盘组织中ANG定量明显高于正常妊娠孕妇(P<0.05);免疫组化法显示ANG主要定位于凶险性前置胎盘孕妇及正常妊娠孕妇胎盘滋养叶细胞的细胞膜和细胞浆中,凶险性前置胎盘孕妇组胎盘组织中ANG表达较正常妊娠孕妇增强,阳性率更高;凶险性前置胎盘孕妇血清ANG浓度与胎盘组织中ANG定量呈正相关(r=0.244,P<0.05),正常妊娠孕妇血清ANG浓度与胎盘组织中ANG定量无相关性(r=0.158,P>0.05)。结论ANG在凶险性前置胎盘孕妇与正常妊娠孕妇中的差异表达可能参与凶险性前置胎盘的发生过程。Objective To explore the role of angiogenin in pernicious placenta previa through detecting the difference expression of angiogenin in pernicious placenta previa pregnant women and normal pregnant women. Methods The expression of angiogenin was detected by ELISA, RT-PCR and immunohistochemistry in 20 cases of pernicious placenta previa and 15 cases of normal pregnant women. Results The level of angiogenin in serum of patients with pernicious placenta previa was significantly higher than that in normal pregnant women(P<0.05);The level of angiogenin in placental tissue of pregnant women with pernicious placenta previa was significantly higher than that in normal pregnant women(P<0.05);immunohistochemical analysis showed that angiogenin was mainly located in the membrane and cytoplasm of placental trophoblast cells in all pregnant women, the expression of angiogenin in pregnant women with pernicious placenta previa was higher than that in normal pregnant women;there was a positive correlation between serum angiogenin and placental tissue in pregnant women with pernicious placenta previa(r=0.244, P<0.05), but no correlation between serum angiogenin and placental tissue in normal pregnant women(r=0.158, P>0.05). Conclusion The differential expression of angiogenin in pregnant women with pernicious placenta previa and normal pregnant women may be involved in pathophysiological process of the development of pernicious placenta previa.

关 键 词：血管生成素 凶险性前置胎盘 胎盘植入 

分 类 号：R714.4[医药卫生—妇产科学]