土壤有效元素含量与延胡索(Corydalis yanhusuo)品质的相关性研究  

Study on the correlation between the content of soil available elements and the quality of Corydalis yanhusuo

作　　者：刘焱 黄小兰 钟志松 曾莹[3] 焦青 周浓 祁俊生 吴应梅 LIU Yan;HUANG Xiaolan;ZHONG Zhisong;ZENG Ying;JIAO Qin;ZHOU Nong;QI Junsheng;WU Yingmei(Laboratory for Green Cultivation and Deep Processing of Three Gorges Reservoir Area’s Medicinal Herbs,College of Life Science and Food Engineering,Chongqing Three Gorges University,Chongqing 404120,China;Chengdu Hehe Medical Laboratory Limited Liability Company,Chengdu 610101,China;Department of Pharmacy,West China Hospital,Sichuan University,Chengdu 610041,China)

机构地区：[1]重庆三峡学院生物与食品工程学院,三峡库区道地药材绿色种植与深加工重庆市工程实验室,重庆404120 [2]成都和合医学检验所有限公司,成都610101 [3]四川大学华西医院药剂科,成都610041

出　　处：《生态科学》2022年第2期108-113,共6页Ecological Science

基　　金：重庆市技术创新与应用示范重点研发项目(cstc2018jscx-mszdX0093);重庆市技术创新与应用示范一般项目(cstc2018jscx-msybX0370);重庆市自然科学基金联合实施项目(cstc2018jcyjAX0770);重庆市教委青年项目(KJQN201801216)。

摘　　要：延胡索乙素是相关延胡索(Corydalis yanhusuo)品质的重要化学成分,在中国药典中作为延胡索质量控制指标,分析不同产地延胡索中延胡索乙素含量与根际土壤有效性元素含量的相关性,对指导延胡索合理化种植栽培有重要科学意义。采用土壤农化法对不同产地延胡索根际土壤中10种元素(大量元素N、P、K、Ca、Mg;微量元素Zn、Mn、Fe、Cu、B)有效性含量进行了测定,同时采用高效液相色谱法(High Performance Liquid Chromatography,HPLC)测定不同产地延胡索中延胡索乙素含量。结果表明:不同产地延胡索根际土壤中有效性元素含量存在显著性差异(P<0.05)。大量元素中,重庆市开县(S2)产地根际土壤有效磷、有效钙含量最高,浙江省东阳市(S4)产地根际土壤有效钾、有效镁含量最高;微量元素中,陕西省汉中市(S3)、浙江省磐安县(S5)产地根际土壤有效铁、有效铜含量显著高于其他产地。不同产地延胡索块茎中的延胡索乙素含量均达0.07%以上,高于药典中的限定标准。相关分析结果表明,延胡索乙素含量与有效钙含量存在极显著正相关(r=0.97,P<0.01),与有效铁存在显著负相关(r=-0.87,P<0.05),延胡索乙素含量主要受根际土壤有效钙含量影响。Tetrahydropalmatine is an important chemical component related to the quality of Corydalis yanhusuo,which is used as a quality control index of Corydalis yanhusuo in Chinese pharmacopoeia.Thus,to analyze the correlation between tetrahydropalmatine content and the content of available elements in rhizosphere soil is a great scientific significance to guide the rational cultivation of Corydalis yanhusuo.The available contents of 10 elements(major elements:N,P,K,Ca,Mg;trace elements:Zn,Mn,Fe,Cu,B)in rhizosphere soil of Corydalis yanhusuo from different producing areas were determined by soil agrochemical method,and the content of tetrahydropalmatine in Corydalis yanhusuo was performed by high performance liquid chromatography(HPLC)in the study.The results showed that there were significant differences in the content of available elements in rhizosphere soil of Corydalis yanhusuo from different producing areas(P<0.05).The contents of available P and Ca in rhizosphere soil of Kaixian Chongqing(S2),as well as the contents of available K and Mg in rhizosphere soil of Dongyang Zhejiang(S4)were highest of all producing areas.Among trace elements,the contents of available iron and copper in rhizosphere soil of Hanzhong Shanxi(S3)and Pan'an Zhejiang(S5)were higher than that of other producing areas.Furthemore,the content of tetrahydropalmatine in Corydalis yanhusuo tuber was above 0.07%,which was higher than the limit standard in Pharmacopoeia.In addition,correlation analysis showed that the content of tetrahydropalmatine was positively related to the available Ca(r=0.97,P<0.01),while negatively related to the available Fe(r=-0.87,P<0.05),and the content of tetrahydropalmatine was mainly affected by the content of available Ca in rhizosphere soil.

关 键 词：延胡索 不同产地 有效性元素 延胡索乙素 

分 类 号：R282.2[医药卫生—中药学]