非酒精性脂肪肝与骨质疏松间的生物信息学关系  

Bioinformatics relationship between non-alcoholic fatty liver and osteoporosis

作　　者：于洪杰 谭国庆[2] YU Hongjie;TAN Guoqing(The People′s Hospital of Cangzhou City, Cangzhou 061000, China;Shandong Hospital of Traditional Chinese Medicine, Jinan 250014, China)

机构地区：[1]沧州市人民医院,河北沧州061000 [2]山东中医药大学附属医院脊柱骨科,山东济南250014

出　　处：《中国骨质疏松杂志》2022年第4期522-526,535,共6页Chinese Journal of Osteoporosis

基　　金：曹贻训全国名老中医传承工作室建设项目(国中医药人教函[2018]134号);徐展望山东省名老中医药专家传承工作室建设项目(山东省卫生健康委员会,鲁卫函[2019]92号)。

摘　　要：目的通过生物信息学相关技术方法依托相关数据库探讨非酒精性脂肪肝与骨质疏松之间的相互影响机制。方法分别以骨质疏松及非酒精性脂肪肝为关键词在相关疾病数据库(Disgenet、TTD、OMIM、Drugbank、Genecards)中筛选相关基因,去重整合后将两组预测靶基因进行映射得到关键靶点,通过STRING网站获得靶点PPI互相作用网络,根据degree值筛选出核心靶点并通过DAVID数据库进行GO富集分析以及KEGG通路分析。结果通过相关疾病数据库搜索筛选出与骨质疏松症和非酒精性脂肪肝相关靶点分别为875个和952个,映射出关键靶点217个,以degree≥36筛选出核心靶点24个并导入DAVID数据库获得78项GO富集结果(P<0.05)及78条KEGG信号通路(P<0.05)。结论IL-6、INS、VEGFA、AKT1等核心靶点作用于乙肝通路、类风湿关节炎通路、TNF通路、IBD通路调控炎症反应及糖脂代谢等生物过程,使骨质疏松及非酒精性脂肪肝互相影响,为临床治疗两种疾病提供了较为科学的理论基础。Objective To explore the interaction mechanism between non-alcoholic fatty liver and osteoporosis through bioinformatics-related technologies and method based on related databases.Methods Used osteoporosis and non-alcoholic fatty liver as keywords,the relevant genes were screened in related disease databases(Disgenet,TTD,OMIM,Drugbank,Genecards),and the two groups of predicted target genes were mapped after de-reintegration to obtain key targets Point,obtained the target PPI interaction network through the STRING website,screened out the core targets according to the degree value,and performed GO enrichment analysis and KEGG pathway analysis through the DAVID database.Results 875 and 952 targets related to osteoporosis and non-alcoholic fatty liver were screened by searching the related disease database.217 key targets were mapped out,and 24 core targets were screened out with degree≥36.Imported DAVID database to obtain 78 GO enrichment result(P<0.05)and 78 KEGG signal pathways(P<0.05).Conclusion IL6,INS,VEGFA,AKT1 and other core targets act on the hepatitis B pathway,rheumatoid arthritis pathway,TNF pathway,IBD pathway to regulate inflammatory response and glucose and lipid metabolism and other biological processes,so that osteoporosis and non-alcoholic fatty liver can interact with each other.The impact provides a more scientific theoretical basis for the clinical treatment of the two diseases.

关 键 词：骨质疏松 非酒精性脂肪肝 生物信息学 糖脂毒性 

分 类 号：R285.5[医药卫生—中药学]