CD44与CA125与老年结核性胸膜炎感染患者体内的炎症免疫关系  

Relationship between CD44 and CA125 and inflammatory immunity in elderly patients with tuberculous pleurisy infection

作　　者：张海燕[1] 范晖[2] 张偲[1] 王永勤[3] ZHANG Haiyan;FAN Hui;ZHANG Si;WANG Yongqin(Department of Infection,Rugao People's Hospital,Rugao,Jiangsu,China,226500;Department of Oncology,Rugao People's Hospital,Rugao,Jiangsu,China,226500;Department of Infectious Diseases,Weifang People's Hospital,Weifang,Shandong,China,261041)

机构地区：[1]江苏省如皋市人民医院感染科,江苏如皋226500 [2]江苏省如皋市人民医院肿瘤科,江苏如皋226500 [3]山东省潍坊市人民医院感染性疾病科,山东潍坊261041

出　　处：《分子诊断与治疗杂志》2022年第3期463-466,470,共5页Journal of Molecular Diagnostics and Therapy

基　　金：2019年度南通市市级科技计划(指导性)立项项目(MSZ19070)。

摘　　要：目的探讨黏附分子(CD44)与糖链抗原-125(CA125)与老年结核性胸膜炎感染患者体内的炎症免疫关系。方法选择2015年7月至2020年7月江苏省如皋市人民医院住院的结核性胸膜炎感染患者120例为观察组,选取同期120例健康体验者作为对照组,对患者的CD44、PBMC RNA进行检测;同时对THP-1细胞培养,并分化为巨噬细胞,进行CFU值统计。分离患者胸水,进行巨噬细胞的刺激试验;并检测CA-125水平。结果外周血PBMC中,经检测观察组的CD44、CD44mRNA水平显著高于对照组,差异有统计学意义(P<0.05)。结合杆菌感染的CD44中加入巨噬细胞后,3周后计算CFU,其结核杆菌载量(1.12±0.02)相较于对照组差异无统计学意义(P<0.05)。加入巨噬细胞前,观察组各指标显著高于对照组,差异有统计学意义(P<0.05),加入后,观察组IL-10指标较对照组,差异无统计学意义(P>0.05),TNF-α水平显著高于对照组(P<0.05),加入TNF-α和抗体后,观察组CD44水平显著低于对照组(P<0.05)。治疗前,观察组CA-125水平显著高于对照组,差异有统计学意义(P<0.05)。CD44与CA125与老年结核性胸膜炎感染患者体内的炎症免疫呈正相关(P<0.05)。结论结核性胸膜炎患者的CD44与CA-125表达与体内的炎症免疫反应变化存在一定的关联。Objective To investigate the relationship between CD44 and CA125 and inflammatory immunity in senile patients with tuberculous pleurisy infection.Methods A total of 120 patients with tuberculous pleurisy infection in Rugao People’s Hospital of Jiangsu Province from July 2015 to July 2020 were selected for this study.A total of 120 healthy subjects were selected as the control group CD44 and PBMCRNA were detected in the patients.At the same time,THP-1 cells were cultured and differentiated into macrophages,and CFU values were counted.The pleural effusion was isolated and macrophage stimulation test was performed.CA-125 level was detected.Results In peripheral blood PBMC,the mRNA level of CD44 and CD44 in the observation group were significantly higher than those in the control group,with statistical significance(P<0.05).After macrophages were added to CD44 infected with Mycobacterium conjugate,CFU was calculated after 3 weeks,and there was no statistically significant difference in mycobacterium tuberculosis load(1.12±0.02)compared with the control group(P<0.05).Before the addition of macrophages,indicators in the observation group were significantly higher than those in the control group,the difference was statistically significant(P<0.05).After the addition of macrophages,IL-10 in the observation group were not statistically significant(P>0.05),and TNF-αlevel was significantly higher than that in the control group,P<0.05.After the addition of TNF-αand antibodies,the level of CD44 in observation group was significantly lower than that in the control group,P<0.05.Before treatment,the level of CA-125 in the observation group was significantly higher than that in the control group,the difference was statistically significant(P<0.05).CD44 and CA125were positively correlated with inflammation and immunity in senile patients with tuberculous pleurisy infection(P<0.05).Conclusion The expression of CD44 and CA-125 in patients with tuberculous pleurisy is correlated with the changes of inflammatory immune response in

关 键 词：结核性胸膜炎 感染 炎症免疫 关系研究 

分 类 号：R521.7[医药卫生—内科学]