碳青霉烯类耐药肠杆菌目细菌检测及鉴定现状  

Current status of detection and identification of carbapenem-resistant Enterobacterales

作　　者：雷静 刘泽世[1] 高兴辉 汤一苇 耿燕[1] LEI Jing;LIU Zeshi;GAO Xinghui;TANG Yiwei;GENG Yan(Department of Laboratory,the Second Affiliated Hospital of Xi’an Jiaotong University,Shannxi,Xi􀆳an,China,710004;Cepheid(Shanghai)Commerce and Trade Co.,Ltd,Shanghai,China,200030)

机构地区：[1]西安交通大学第二附属医院检验科,陕西西安710004 [2]赛沛(上海)商贸有限公司,上海200030

出　　处：《分子诊断与治疗杂志》2022年第3期533-538,共6页Journal of Molecular Diagnostics and Therapy

基　　金：陕西省重点研发计划(2017SF-128)。

摘　　要：碳青霉烯类耐药肠杆菌目细菌(CRE)感染在国内迅速蔓延,临床实验室及时检测CRE对院感防控至关重要。产生碳青霉烯酶是CRE主要的耐药机制。目前CRE实验室检测包括表型和基因型方法。表型方法通过碳青霉烯酶对碳青霉烯类药物的水解作用或抑制剂对酶的抑制作用来检测其活性,或通过添加底物筛选出CRE有色菌落。基因型方法主要包括靶向基因检测和二代测序,其检测编码碳青霉烯酶的DNA序列。本文对CRE检测方法及鉴定现状进行研究。Carbapenem-resistant Enterobacterales(CRE)infection spreads rapidly in China.Timely detection of CRE in clinical laboratory is very important for nosocomial infection prevention and control.In CRE,carbapenemases are the main contributing resistance mechanism.At present,CRE detection include phenotypic and genotype methods in the laboratory.Phenotypic methods detect the activity of carbapenemases through the hydrolysis of carbapenemases on carbapenem drugs or inhibiting carbapenemase activity by inhibitors,or screens colored CRE colonies by adding substrates.Genotype methods include targeted gene detection and next-generation sequencing,which detect the encoding carbapenemase DNA sequence.In this paper,the detection and identification methods of CRE were studied retrospectively.

关 键 词：碳青霉烯类耐药肠杆菌目细菌 碳青霉烯酶 表型检测 基因型检测 

分 类 号：R446.5[医药卫生—诊断学]