鼻咽癌组织中c-IAP2的表达及其临床意义  

Expression of c-IAP2 in Nasopharyngeal Carcinoma and Its Clinical Significance

作　　者：谢桂华[1] 罗会舟 XIE Guihua;LUO Huizhou(Department of Otorhinolaryngology,Liyuan Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology,Wuhan,430077,China)

机构地区：[1]华中科技大学同济医学院附属梨园医院耳鼻喉科,武汉市430077

出　　处：《医学分子生物学杂志》2022年第2期110-114,共5页Journal of Medical Molecular Biology

摘　　要：目的探究细胞凋亡抑制蛋白2(cellular inhibitor of apoptosis protein 2,c-IAP2)在鼻咽癌组织中的表达以及临床意义。方法回顾性分析95例鼻咽癌患者的肿瘤组织和40例正常患者的鼻咽组织及临床预后资料,分别利用RT-PCR、Western印迹以及免疫组织化学染色检测上述组织中c-IAP2 mRNA和蛋白的表达水平;卡方检验探究肿瘤组织中c-IAP2表达与临床病理学特征的关系;采用Kaplan-Meier结合Logrank检验表达不同c-IAP2表达水平患者的生存差异;采用单因素和多因素Cox比例风险回归模型分析影响鼻咽癌患者预后的危险因素。结果c-IAP2 mRNA及蛋白水平在鼻咽癌组织中显著高于正常组织(t=12.62,P<0.001);进一步,免疫组化分析发现c-IAP2在鼻咽癌组织呈高表达,且其高表达与肿瘤T分期、N分期、TNM分期及分化程度显著相关(P均<0.05);生存分析显示c-IAP2低表达组鼻咽癌患者的总生存率显著高于高表达组(χ^(2)=11.86,P=0.001),且c-IAP2高表达是导致鼻咽癌患者总生存期缩短的一个独立危险因素(HR=2.425,95%CI:1.256~4.994,P=0.012)。结论鼻咽癌中c-IAP2的表达水平明显上调,其高表达与患者的不良预后显著相关,对于评估鼻咽癌患者的预后具有潜在的临床应用价值。Objective To explore the expression and clinical significance of cellular inhibitor of apoptosis protein 2(c-IAP2)in nasopharyngeal carcinoma(NPC).Methods The NPC tissues and the nasopharyngeal normal tissues were obtained from 95 NPC patients and 40 non-NPC patients,respectively,and their clinico-prognostical data were analyzed.The expression of c-IAP2 mRNA and protein in these tissues was detected by RT-PCR,immunochemistry and Western blotting,respectively.The relationship between the clinicopathological characteristics and c-IAP2 expression was analyzed by the chi-square test.Kaplan-Meier combined with Log-rank was used to test the survival difference of patients with different expression levels of c-IAP2.Moreover,univariate and multivariate Cox proportional risk regression models were used to analyze the risk factors affecting the prognosis of NPC patients.Results RT-PCR showed that the mRNA expression level of cIAP2 was significantly increased in NPC tissues when compared with normal tissues(t=12.62,P<0.001).Western blotting analysis showed that the expression level of c-IAP2 protein was significantly up-regulated in NPC tissues,which was consistent with the change of c-IAP2 mRNA expression level.Furthermore,the immunohistochemical analysis displayed that c-IAP2 was highly expressed in NPC tissues,and its expression level was associated with tumor differentiation,T staging,N staging,and TNM staging(P<0.05,respectively).Survival analysis found that the overall survival rate in NPC patients with low c-IAP2 expression level was significantly higher than that in NPC patients with high c-IAP2 expression level(χ^(2)=11.86,P=0.001),and the high expression of cIAP2 was an independent risk factor for shortened overall survival of patients with NPC(HR=2.425,95%CI:1.256~4.994,P=0.012).Conclusion The expression level of c-IAP2 in NPC is significantly up-regulated,and its high expression is significantly correlated with the poor prognosis of NPC patients,which has potential clinical value for evaluating the prognosis of

关 键 词：鼻咽癌 C-IAP2 临床病理特征 预后 

分 类 号：R762[医药卫生—耳鼻咽喉科]