西沙乳白肉芝软珊瑚中的化学成分及其抗炎活性研究  

Chemical constituents of soft coral Sarcophyton glaucum collected from Xisha and their anti-inflammatory activity

作　　者：李金凤 姚励功 曾艳波 郭跃伟[2,4] LI Jin-feng;YAO Li-gong;ZENG Yan-bo;GUO Yue-wei(Ocean College of Hebei Agricultural University,Qinhuangdao 066000,China;State Key Laboratory of Drug Research,Shanghai Institute of Materia Medica,Chinese Academy of Sciences,Shanghai 201203,China;Hainan Provincial Key Laboratory for Functional Components Research and Utilization of Marine Bio-resources,Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China;Bohai rim Advanced Research Institute for Drug Discovery,Yantai 264000,China)

机构地区：[1]河北农业大学海洋学院,河北秦皇岛066000 [2]中国科学院上海药物研究所新药研究国家重点实验室,上海201203 [3]中国热带农业科学院热带生物技术研究所海南省海洋生物资源功能性成分研究与利用重点实验室,海南海口571101 [4]中科环渤海(烟台)药物高等研究院,山东烟台264000

出　　处：《药学学报》2022年第3期741-749,共9页Acta Pharmaceutica Sinica

基　　金：海南省重点研发计划项目(ZDYF2021SHFZ107);国家自然科学基金项目(81991521);新药研究国家重点实验室项目(SIMM2103ZZ-06);农业农村部财政专项项目(NFZX2021)。

摘　　要：综合运用硅胶柱色谱、凝胶(Sephadex-20)柱色谱、薄层色谱、半制备HPLC等多种色谱技术对中国南海西沙采集到的乳白肉芝软珊瑚Sarcophyton glaucum的乙醚相进行分离纯化,共分离得到26个化合物。根据其理化性质和各种波谱数据分析,分别鉴定为:15-dehydroxy-sarcophytol D (1)、ximaoglaucumin C (2)、(11S,12S,1E,3E,7E)-11,12-epoxycembra-1,3,7-triene (3)、sarcophytol W (4)、cembrene (5)、sarcophytol B (6)、sarcophytol K (7)、sarcophytol J(8)、pentaene-cembrene (9)、sarcophytol E (10)、(+)-marasol (11)、(2S)-sarcophytoninsarcophytoxide (12)、(-)-17-hydroxydeepoxysarcophytoxide (13)、(+)-sarcophytoxide (14)、13-acetoxysarcophytoxide (15)、bophynin B (16)、16-oxosarcophytoxide E (17)、sarcophinone (18)、7α-8β-dihydroxydeepoxysarcophine (19)、(+)-sarcophine (20)、14-dehydroxy-sarcophytol L (21)、sarcophytol L (22)、13α-hydroxy-sarcophytol L (23)、trocheliophol C (24)、trocheliophol E(25)和trocheliophol L (26)。其中化合物1为新的西松烷型二萜。抗炎生物活性测试结果表明,化合物4对脂多糖(LPS)诱导的BV2细胞炎症反应具有抑制作用。The crude Et;O extract of soft coral Sarcophyton glaucum, collected off the Xisha, the South China Sea, were investigated. A new cembrane-type diterpenoid, namely 15-dehydroxy-sarcophytrol D(1), together with twenty-five known compounds, namely ximaoglaucumin C(2),(11S,12S,1E,3E,7E)-11,12-epoxycembra-1,3,7-triene(3), sarcophytol W(4), cembrene(5), sarcophytol B(6), sarcophytol K(7), sarcophytol J(8), pentaene-cembrene(9), sarcophytol E(10),(+)-marasol(11),(2S)-sarcophytoninsarcophytoxide(12),(-)-17-hydroxydeepoxysarcophytoxide(13),(+)-sarcophytoxide(14), 13-acetoxysarcophytoxide(15), bophynin B(16), 16-oxosarcophytoxide E(17), sarcophinone(18), 7α-8β-dihydroxydeepoxysarcophine(19),(+)-sarcophine(20), 14-dehydroxy-sarcophytol L(21), sarcophytol L(22), 13α-hydroxy-sarcophytol L(23), trocheliophol C(24), trocheliophol E(25) and trocheliophol L(26), were isolated and purified by comprehensive chromatography methods of silica gel column, Sephadex LH-20 gel column, TLC, and semi-preparative high-performance liquid chromatography(HPLC). In anti-inflammatory bioassay, compound 4 exhibited inhibitory effects on lipopolysaccharide(LPS)-induced inflammatory responses in BV-2 microglial cells.

关 键 词：软珊瑚 乳白肉芝软珊瑚 二萜 15-dehydroxy-sarcophytol D 抗炎活性 

分 类 号：R284[医药卫生—中药学]