基于液相色谱及网络药理学的滇龙胆质量标志物(Q-marker)预测分析  

Q-marker prediction analysis of Gentiana rigescens Franch. based on HPLC and network pharmacology

作　　者：刘春路 沈涛[2,3] 张霁 徐福荣[1] 王元忠 LIU Chun-lu;SHEN Tao;ZHANG Ji;XU Fu-rong;WANG Yuan-zhong(College of Traditional Chinese Medicine,Yunnan University of Chinese Medicine,Kunming 650500,China;Institute of Medicinal Plants,Yunnan Academy of Agricultural Sciences,Kunming 650200,China;College of Chemistry,Biology and Environment,Yuxi Normal University,Yuxi 653100,China)

机构地区：[1]云南中医药大学中药学院,云南昆明650500 [2]云南省农业科学院药用植物研究所,云南昆明650200 [3]玉溪师范学院化学生物与环境学院,云南玉溪653100

出　　处：《药学学报》2022年第3期766-774,共9页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金资助项目(82060690,32060086)。

摘　　要：滇龙胆(Gentiana rigescens Franch.)是我国的特色药用植物,具有抗炎、保肝等功效。目前,对于滇龙胆特征成分的含量测定方面研究较少,因此有必要建立科学有效的质量控制方法;本研究建立了高效液相色谱(HPLC)指纹图谱,通过文献查阅及特征图谱指认,筛选滇龙胆质量标志物(Q-marker)的来源范围。网络药理学分析成分的有效性及其所对应的靶点和通路,绘制“成分-靶点-通路”图。HPLC定性和定量分析筛选组间差异的主要标志成分,最终确定滇龙胆的质量标志物;文献和HPLC特征图谱确定了5种环烯醚萜类成分为滇龙胆质量标志物的主要来源范围。多产地滇龙胆的网络药理学(有效性)和定性定量(检测性)分析确定獐芽菜苷、龙胆苦苷、獐芽菜苦苷3种成分可作为主要标志性成分,且不同产地间成分含量差异显著;通过网络药理学和指纹图谱对不同产地滇龙胆进行分析,确定可作为其潜在质量标志物的成分,为后期滇龙胆的质量控制提供充分的理论依据。Aa a characteristic medicinal plant in China, Gentiana rigescens Franch. has the function of protecting the liver and invigorating the spleen. At present, there are a few studies on the content determination method of characteristic components of G. rigescens, so it is necessary to establish a scientific and effective quality control method;In this study, The high performance liquid chromatography(HPLC) fingerprint of G. rigescens was established, based on literature reviewed and characteristic spectrum identified, the source range of G. rigescens quality marker(Q-marker) was screened. The effectiveness of the ingredients and the corresponding targets and pathways was analyzed through network pharmacology, and drew the diagram of ’’component-target-pathway’’.Qualitative and quantitative analysis of G. rigescens was performed by HPLC, and screen the main marker components leading to the differences between groups which were determined the Q-marker of G. rigescens;The literature and HPLC had determined that five iridoids were the main source of G. rigescens Q-marker. The network pharmacology(effectiveness) and qualitative and quantitative(detectability) analysis of G. rigescens from different producing areas confirmed that gentiopicroside, swertiamarin, and sweroside can be used as the main landmark components, and there were significant differences in their contents among different producing areas;The analysis of G. rigescens from different producing areas was carried out by network pharmacology and chemical fingerprints, it is confirmed can be used as potential Q-marker to provide sufficient theoretical basis for the quality control of G. rigescens in the later period.

关 键 词：滇龙胆 质量标志物 网络药理学 化学指纹图谱 化学成分 

分 类 号：R917[医药卫生—药物分析学]