基于Heracles NEO超快速气相色谱电子鼻对温郁金醋制前后气味差异标志物的快速识别研究  

Rapid identification of odor difference markers in Curcuma wenyujin Rhizoma before and after vinegar with processing based on ultrafast gas chromatography electronic nose

作　　者：秦宇雯 费程浩 苏联麟 季德[1] 李昱 张伟[1,2] 姜梁钰 毛春芹 陆兔林[1,3] QIN Yu-wen;FEI Cheng-hao;SU Lian-lin;JI De;LI Yu;ZHANG Wei;JIANG Liang-yu;MAO Chunqin;LU Tu-lin(School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China;School of Pharmacy,Anhui University of Chinese Medicine,Hefei 230012,China;Jiangsu Provincial Engineering Research Center of TCM External Medication Development and Application,Nanjing 210023,China)

机构地区：[1]南京中医药大学药学院,江苏南京210023 [2]安徽中医药大学药学院,安徽合肥230012 [3]江苏省中医外用药开发与应用工程研究中心,江苏南京210023

出　　处：《中草药》2022年第5期1313-1319,共7页Chinese Traditional and Herbal Drugs

基　　金：国家重点研发计划项目(2018YFC1707000);国家自然科学基金项目(81973483)。

摘　　要：目的基于Heracles NEO超快速气相色谱电子鼻对温郁金醋制前后气味差异标志物进行快速识别研究,拟建立一种基于气味的有效快速识别方法。方法通过建立生、醋温郁金的Heracles NEO检测方法,结合Arochembase数据库,采用主成分分析(principal component analysis,PCA)、判别因子分析(discriminant factor analysis,DFA)和变量投影重要性指标(variable importance in projection,VIP)方法,对其色谱峰进行数据处理和分析。结果经Heracles NEO检测,温郁金共18个色谱峰,利用Arochembase数据库表征出17个气味成分,采用PCA、DFA均能将生、醋温郁金准确区分,通过OPLSDA模型中VIP值分析,在MXT-5和MXT-1701色谱柱下,5、6、10号色谱峰的VIP均>1.0,推测γ-松油烯、芳樟醇、乙酸癸酯是生、醋温郁金的潜在气味差异标志物。结论Heracles NEO可应用于生、醋温郁金的气味差异标志物快速识别,具有重要的应用价值。Objective In order to establish an effective and fast recognition method based on odor,Heracles Neo ultra-fast gas chromatography electronic nose was used to study the rapid identification of odor difference markers in Curcuma wenyujin Rhizoma before and after vinegar with processing.Methods Through the establishment of Heracles Neo detection method for raw Curcuma wenyujin Rhizoma pieces and vinegar Curcuma wenyujin Rhizoma pieces,combined with arochembase database,principal component analysis(PCA),discriminant factor analysis(DFA)and variable importance in the projection(VIP)were used to process and analyze the chromatographic peak data.Results According to Heracles Neo,there were 18 chromatographic peaks in Curcuma wenyujin Rhizoma pieces,17 odor components were characterized by arochembase database.PCA and DFA could distinguish raw and vinegar Curcuma wenyujin Rhizoma pieces accurately.Through the analysis of VIP value in opls-da model,the VIP of No.5,No.6 and No.10 chromatographic peaks were>1.0 under MXT-5 and MXT-1701 chromatographic columns.Therefore,it was speculated thatγterpinene,linalool and decyl acetate are potential odor markers of raw and vinegar Curcuma wenyujin Rhizoma pieces.Conclusion Heracles Neo can be applied to the rapid identification of odor difference markers in raw and vinegar Curcuma wenyujin Rhizoma pieces,which has important application value.

关 键 词：温郁金 醋制 Heracles NEO 气味差异标志物 快速识别 γ-松油烯 芳樟醇 乙酸癸酯 

分 类 号：R283.6[医药卫生—中药学]