银花青蒿栀子方抗A6型柯萨奇病毒活性成分的分离  

Isolation of antivial constituents of Yinhua Qinghao-Zhizi Description against Coxsavirus A6

作　　者：李菲 赵新亚 赵雪梅 毕研平 李娟 崔清华[2] 田景振[2] LI Fei;ZHAO Xin-ya;ZHAO Xue-mei;BI Yan-ping;LI Juan;CUI Qing-hua;TIAN Jing-zhen(Key Laboratory of Etiology and Epidemiology of Emerging Infectious Diseases,Department of Pharmacy,Shandong First Medical University&Shandong Academy of Medical Sciences,Taian 271016,China;Department of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,China)

机构地区：[1]山东第一医科大学(山东省医学科学院)药学院,新发传染病病因流行病学重点实验室,山东泰安271016 [2]山东中医药大学药学院,山东济南250355

出　　处：《中草药》2022年第5期1365-1371,共7页Chinese Traditional and Herbal Drugs

基　　金：山东省重点研发计划(2017GSF19106);山东省中医药科技发展计划(2017-248);山东第一医科大学学术提升计划(2019LJ003)。

摘　　要：目的研究银花青蒿栀子方(Yinhua Qinghao Zhizi Description,YQZ)抗A6型柯萨奇病毒(Coxsavirus A6,CV-A6)的活性成分。方法对YQZ进行提取、分离和结构鉴定,以病毒抑制率为指标追踪其抗CV-A6活性成分,利用Discovery Studio2017软件将其与受体蛋白进行分子对接。结果YQZ醇提物的石油醚部位分离得到的青蒿酸和青蒿素、正丁醇部位分离得到的栀子苷、绿原酸和木犀草苷对CV-A6的抑制率显著高于阳性对照利巴韦林(P<0.05、0.01),上述成分分子和CV-A6衣壳蛋白VP1之间均存在多个氢键、范德华引力等作用,亲和力大。结论YQZ中的青蒿酸、青蒿素、栀子苷、绿原酸和木犀草苷具有良好的抗CV-A6活性,可能与抑制病毒衣壳蛋白VP1有关。Objective To explore the active constituents of YQZ(Yinhua Qinghao Zhizi Description,YQZ)against Coxsavirus A6(CV-A6).Methods Active constituents were extracted and isolated from YQZ and identified,which was proceeded with virus inhibition rate as the evaluation index.Molecule docking between active constituents and target protein was performed using Discovery Studio 2017 software.Results Compared with ribavirin,the virus inhibition rate of artemisic acid and artemisinin seperated from petroleum ether extract,and geniposide,chlorogenic acid as well as luteoloside from n-Butanol extract from YQZ ethanol extract was significantly higher(P<0.01,0.05).Dense interaction involving hydrogen bonds and van der waals force,etc.exsited between molecules of the aboved constituents and CV-A6 capsid protein VP1.Conclusion Artemisic acid,artemisinin,geniposide,chlorogenic acid,and luteoloside in YQZ has significant anti-CV-A6 effect,which may be ralated with the inhibition of capsid protein VP1.

关 键 词：银花青蒿栀子方 A6型柯萨奇病毒 抗病毒 活性成分 分子对接 青蒿酸 青蒿素 栀子苷 绿原酸 木犀草苷 

分 类 号：R284.1[医药卫生—中药学]