基于网络药理学的丹红化瘀口服液治疗视网膜中央静脉阻塞症的作用机制研究  

Mechanisms of Danhong Huayu Oral Liquid in treatment of central retinal vein occlusion based on network pharmacology

作　　者：林娟[1] 韩彦琪[2,3] 武琦 许浚[2,3,5] 刘耀晨 张传平[1] 李楚源[1] 张铁军[2,3] LIN Juan;HAN Yan-qi;WU Qi;XU Jun;LIU Yao-chen;ZHANG Chuan-ping;LI Chu-yuan;ZHANG Tie-jun(Guangzhou Baiyunshan Hutchison Whampoa Chinese Medicine Co.,Ltd.,Guangzhou 510515,China;Tianjin Key Laboratory of Quality Marker of Traditional Chinese Medicine,Tianjin Institute of Pharmaceutical Research,Tianjin 300462,China;State Key Laboratory of Drug Delivery Technology and Pharmacokinetics,Tianjin Institute of Pharmaceutical Research,Tianjin 300462,China;Pharmaron Beijing Co.,Ltd.,Beijing 100176,China;Research Unit for Drug Metabolism,Chinese Academy of Medical Sciences,Tianjin Institute of Pharmaceutical Research,Tianjin 300462,China;Tianjin Medical University,Tianjin 300070,China)

机构地区：[1]广州白云山和记黄埔中药有限公司,广东广州510515 [2]天津药物研究院天津市中药质量标志物重点实验室,天津300462 [3]天津药物研究院释药技术与药物代谢动力学国家重点实验室,天津300462 [4]康龙化成(北京)生物技术有限公司,北京100176 [5]天津药物研究院,中国医学科学院药物代谢新技术创新单元,天津300462 [6]天津医科大学,天津300070

出　　处：《中草药》2022年第6期1643-1651,共9页Chinese Traditional and Herbal Drugs

基　　金：广州市珠江科技新星专项资助(201806010082);中国医学科学院医学与健康科技创新工程项目资助(2019-I2M-5-020);广州市科学技术协会2019年“广州市院士专家工作站”建设项目资助(600180)。

摘　　要：目的 基于网络药理学探究丹红化瘀口服液治疗视网膜静脉中央阻塞症的作用机制。方法 选取丹红化瘀口服液中的21个入血成分为研究对象,借助TCMSP和CTD数据库预测化合物的作用靶点,利用MAS 3.0生物分子功能软件获取相关通路,通过FUNRICH软件对获得靶点进行基因本体(gene ontology,GO)功能富集分析,最后利用Cytoscape软件构建“化合物-靶点-通路-疾病”网络图。结果 21个成分可作用于104个蛋白靶点和45条通路,其中多个靶点和通路与视网膜静脉中央阻塞症相关。结论 丹红化瘀口服液通过作用于与凝血过程、新血管生成、血管舒张、免疫调节和炎症反应等相关的靶点,起到活血化瘀、行气通络的功效,从而治疗视网膜中央静脉阻塞症。Objective To study the mechanisms of Danhong Huayu Oral Liquid(丹红化瘀口服液)in the treatment of central retinal vein occlusion(CRVO)by network pharmacology.Methods Twenty-one components absorbed into blood of Danhong Huayu Oral Liquid were selected as objects,targets were explored by TCMSP and CTD database and related pathways were obtained by MAS 3.0 software.The gene ontology(GO)functional analysis were explored by FUNRICH software.Finally,the network of“compoundtarget-pathway-disease”of Danhong Huayu Oral Liquid was constructed by Cytoscape software.Results Twenty-one components of Danhong Huayu Oral Liquid affected 45 pathways through 104 target proteins which were related to CRVO.Conclusion Danhong Huayu Oral Liquid has the effect of promoting blood circulation and removing blood stasis,invigorating qi and activating collaterals through acting on the targets related to coagulation process,neovascularization,vasodilation,immune regulation and inflammatory reaction,thereby treating CRVO.

关 键 词：丹红化瘀口服液 视网膜中央静脉阻塞症 网络药理学 活血化瘀 行气通络 

分 类 号：R285.5[医药卫生—中药学]