基于GC-MS和UPLC-Q-Exactive MS技术的荆防颗粒化学成分研究  

Chemical constituents of Jingfang Granules based on GC-MS and UPLC-Q Exactive MS

作　　者：梁红宝 姜宇 袁晓梅 姚景春 邱荣英 杨敏[1] 张贵民 李峰[1] LIANG Hong-bao;JIANG Yu-jun;YUAN Xiao-mei;YAO Jing-chun;QIU Rong-ying;YANG Min;ZHANG Gui-min;LI Feng(Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Shandong New Time Pharmaceutical Co.,Ltd.,Linyi 276006,China;State Key Laboratory of Generic Manufacture Technology of Chinese Traditional Medicine,Lunan Pharmaceutical Group Co.,Ltd.,Linyi 276006,China)

机构地区：[1]山东中医药大学,山东济南250355 [2]山东新时代药业有限公司,山东临沂276006 [3]鲁南制药集团股份有限公司中药制药共性技术国家重点实验室,山东临沂276006

出　　处：《中草药》2022年第6期1697-1708,共12页Chinese Traditional and Herbal Drugs

基　　金：国家重大新药创制专项(2017ZX09301058)。

摘　　要：目的对荆防颗粒化学成分进行系统分析,并分析其裂解规律。方法采用GC-MS技术对荆防颗粒中挥发性化学成分和UPLC-Q-ExactiveMS技术在正、负离子模式下对荆防颗粒中非挥发性化学成分进行定性分析。结果通过GC-MS数据库匹配,共鉴别出了24个化合物,包括萜类19个、醛类2个、酯类2个、芳香醚类1个。通过质谱信息并结合对照品、相关文献、数据库检索,液质联用共鉴别出了85个化合物,包括香豆素类25个、木脂素类6个、黄酮苷15个、黄酮类15个、有机酸类9个、糖苷类5个、三萜类4个和其他类化合物6个。结论建立了荆防颗粒的GC-MS和UPLC-Q-Exactive MS的分离鉴定方法,为阐释该复方的功效物质基础提供了依据。Objective To investigate the chemical composition of Jingfang Ganules(荆防颗粒),and analyze its fragmentation pattern.Methods The volatile and non-volatile chemical components in Jingfang Granules were qualitatively analyzed by GC-MS and UPLC-Q-Exactive MS in positive and negative ion mode,respectively.Results A total of 24 compounds(19 terpenoids,two aldehydes,two esters and one aromatic ether)were identified by GC-MS.And a total of 85 compounds,(25 coumarins,six lignans,15 flavonoid glycosides,15 flavonoids,nine organic acids,five glycosides,four triterpenoids and six other compounds)were identified by UPLC-Q Exactive MS and combined with reference materials,related literatures and database retrieval.Conclusion The separation and identification methods of Jingfang Granules by GC-MS and UPLC-Q Exactive MS were established,which provided a foundation for further research of pharmacodynamic material basis of it.

关 键 词：荆防颗粒 GC-MS UPLC-Q Exactive MS 裂解规律 萜类 香豆素 木脂素 

分 类 号：R284.1[医药卫生—中药学]