基于UHPLC-ESI-QE-Orbitrap-MS技术的人参固本口服液干预食蟹猴颈椎间盘置换术术后的血清代谢组学研究  被引量:6

Effect of Ginseng Guben Oral Liquid on serum metabolomics in cynomophagous monkeys after cervical disc replacement based on UHPLC-ESI-QE-Orbitrap-MS

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作  者:钟诚 杨东亮 姚景春 孙成宏 晋大祥[1] 孙国栋[4] 张贵民 ZHONG Cheng;YANG Dong-liang;YAO Jing-chun;SUN Cheng-hong;JIN Da-xiang;SUN Guo-dong;ZHANG Gui-min(The First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510006,China;Jiangmen Hospital of Traditional Chinese Medicine Affiliated to Jinan University,Jiangmen 529000,China;State Key Laboratory of Generic Manufacture Technology of Traditional Chinese Medicine,Lunan Pharmaceutical Group Co.Ltd.,Linyi 276006,China;The First Affiliated Hospital of Jinan University,Guangzhou 510630,China)

机构地区:[1]广州中医药大学第一附属医院,广东广州510006 [2]暨南大学附属江门中医院,广东江门529000 [3]鲁南制药集团股份有限公司中药制剂共性技术国家重点实验室,山东临沂276006 [4]暨南大学附属第一医院,广东广州510630

出  处:《中草药》2022年第6期1776-1782,共7页Chinese Traditional and Herbal Drugs

基  金:国家科技重大专项(2018ZX09201010);广东省医学科研基金(A2019217);江门市科技计划项目(2021YL03002)。

摘  要:目的采用代谢组学方法分析人参固本口服液(Ginseng Guben Oral Liquid,RSGB)对食蟹猴人工颈椎间盘置换术后血清代谢产物的影响,探讨人参固本口服液促进猕猴颈椎术后康复的机制。方法 24只食蟹猴根据体质量随机分为正常组、模型组、RSGB组,每组8只。通过食蟹猴人工颈椎间盘置换术制备模型,正常组、模型组ig给予蒸馏水,RSGB组ig给予人参固本口服液,观察食蟹猴术后康复情况。运用超高效液相色谱-四极杆/静电场轨道阱高分辨质谱技术(UHPLC-ESIQE-Orbitrap-MS)检测血清差异代谢物,分析可能的代谢通路。结果 RSGB组食蟹猴一般情况明显改善。代谢组学显示,与正常组相比,模型组食蟹猴结肠共发现乙酰胆碱、烟酰胺、蛋氨酸、乙酰左旋肉碱、次黄嘌呤、鸟嘌呤、肌苷、鸟苷、次黄嘌呤、亮氨酸、异喹啉、脱氧胆酸、胆酸、肉豆蔻酸、棕榈油酸、二十二碳六烯酸、亚油酸、花生四烯酸、肉豆蔻酸乙酯、油酸等23个代谢差异物。RSGB组这些代谢差异物有向正常水平回调的趋势。结论推测RSGB促进食蟹猴颈椎术后康复可能与调控亚油酸代谢、花生四烯酸代谢、色氨酸及半胱氨酸和蛋氨酸代谢等多条代谢通路有关。Objective Metabolomics method was used to analyze the effects of Ginseng Guben Oral Liquid(人参固本口服液,RSGB)on serum metabolites in cynomophagous monkeys after cervical disc replacement.To explore the mechanism of RSGB promoting cervical vertebra postoperative rehabilitation in cynomophagous monkeys.Methods According to body weight,24 cynomophagous monkeys were randomly divided into normal group,model group and RSGB group,with eight monkeys in each group.The models were made by cervical disc replacement in cynomophagous monkeys,normal group and model group were given distilled water intragastrically,RSGB group was given RSGB intragastrically,and the postoperative rehabilitation of cynomolgus monkeys was observed.Ultra-high-performance liquid chromatography electrospray ionization orbitrap tandem mass spectrometry(UHPLC-ESIOrbitrap-MS/MS)was used to detect differential metabolites in blood and analyze possible metabolic pathways.Results The results showed that the general condition of cynomophagous monkeys in RSGB group was significantly improved.Metabonomics showed that compared with the normal group,model group were found colon acetylcholine,niacinamide,methionine,acetyl-L-carnitine,inosine,guanosine,hypoxanthine,leucine,isoquinoline,deoxycholic acid,cholic acid,myristic acid,palmitoleic acid,docosahexaenoic acid,linoleic acid,arachidonic acid,ethyl myristate,oleic acid and other 23 metabolic differentials.In the RSGB group,these metabolic differentials tended to return to the normal level.Conclusion It is speculated that the promotion of RSGB in postoperative rehabilitation of cervical vertebra in cynomophagous monkeys may be related to the regulation of linoleic acid metabolism,arachidonic acid metabolism,tryptophan,cysteine and methionine metabolism and other metabolic pathways.

关 键 词:人参固本口服液 颈椎间盘置换术 代谢差异物 代谢通路 食蟹猴 亚油酸代谢通路 花生四烯酸代谢通路 

分 类 号:R285[医药卫生—中药学]

 

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