黄体酮对雄性切口痛大鼠机械缩足反射阈值、脊髓神经激肽‑1受体及血浆P物质表达的影响  

Effects of progesterone on paw mechanical withdraw threshold,spinal neurokinin⁃1 receptor and plasma substance P expres⁃sion in male rats with incisional pain

作　　者：苏凯 缪慧慧 田鸣[1] 薛富善 Su Kai;Miao Huihui;Tian Ming;Xue Fushan(Department of Anesthesiology,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China;Department of Anesthe-siology,Beijing Shijitan Hospital,Capital Medical University,Beijing 100038,China)

机构地区：[1]首都医科大学附属北京友谊医院麻醉科,北京100050 [2]首都医科大学附属北京世纪坛医院麻醉科,北京100038

出　　处：《国际麻醉学与复苏杂志》2022年第3期231-234,共4页International Journal of Anesthesiology and Resuscitation

基　　金：北京市自然科学基金(7214221)。

摘　　要：目的观察黄体酮对切口痛大鼠机械缩足反射阈值(paw mechanical withdraw threshold,PMWT)、脊髓神经激肽‑1受体(neurokinin‑1 receptor,NK‑1R)、血浆P物质表达的影响,探讨黄体酮对疼痛影响的可能机制。方法采用随机数字表法将24只健康成年雄性Wistar大鼠分为对照组(C组)、黄体酮组(P组,术前3 h肌内注射1.5 mg/100 g)、米非司酮组(M组,术前3 h灌胃1.5 mg/100 g),每组8只。按照Brennan法制备足底切口痛模型,测定大鼠给药前(T_(0))、手术前(T_(1))、手术后1 h(T_(2))、手术后3 h(T_(3))4个时间点的术侧足PMWT。在T_(3)时间点采用免疫印迹法测定脊髓NK‑1R水平,用ELISA法测定血浆P物质水平。结果与C组比较,P组和M组T_(2)、T_(3)时PMWT升高(P<0.05),且P组PMWT高于M组(P<0.05)。与C组比较,P组和M组T_(3)时脊髓NK‑1R及血浆P物质水平减低(P<0.05),且P组脊髓NK‑1R及血浆P物质水平低于M组(P<0.05)。结论黄体酮能够抑制切口疼痛,可能与黄体酮受体结合状态抑制脊髓NK‑1R的表达及P物质生成和(或)释放有关。Objective To observe the effects of progesterone on the paw mechanical withdraw threshold(PMWT),spinal cord neurokinin‑1 receptor(NK‑1R),and plasma substance P expression in rats with incisional pain,and to explore the possible mechanism of progesterone on the pain.Methods According to the random number table method,24 healthy adult male Wistar rats were divided into three groups(n=8):a control group(group C),a progesterone group(group P,intramuscular injection of 1.5 mg/100 g 3 h before op‑eration),and a mifepristone group(group M,intragastric administration of 1.5 mg/100 g 3 h before operation).An paw incisional pain model was established according to Brennan's method,and the PMWT of the injured paw was measured at four time points:before ad‑ministration(T_(0)),before operation(T_(1)),1 h after operation(T_(2)),and 3 h after operation(T_(3)).Western blot was used to detect the level of NK‑1R in the spinal cord,and enzyme‑linked immunosorbent assay(ELISA)was used to determine plasma substance P at T_(3).Re⁃sults Compared with that in group C,PMWT in group P and group M increased at T_(2) and T_(3)(P<0.05),and PMWT in group P was higher than that in group M(P<0.05).Compared with group C,the levels of spinal cord NK‑1R and plasma substance P at T_(3) in group P and group M decreased(P<0.05),and the levels of spinal cord NK‑1R and plasma substance P in group P were lower than those in group M(P<0.05).Conclusions Progesterone can inhibit incision pain,which may be related to its binding with the progesterone re‑ceptor to inhibit the expression of NK‑1R and the production and(or)release of substance P.

关 键 词：黄体酮 切口痛 P物质 神经激肽‑1受体 

分 类 号：R614[医药卫生—麻醉学]