飞行时间质谱-GC-MS/MS法测定桂花中β-紫罗兰酮  

Determination ofβ-Ionone in Osmanthus fragrans(Thunb.)Lour.by GC-Q-TOF MS and GC-MS/MS

作　　者：魏小华 章嘉晴 魏竹秋 WEI Xiao-hua;ZHANG Jia-qing;WEI Zhu-qiu(Jiangsu Province Wuxi Environmental Monitoring Central,Wuxi,Jiangsu 214000,China)

机构地区：[1]江苏省无锡环境监测中心,江苏无锡214000

出　　处：《环境监测管理与技术》2022年第2期50-52,共3页The Administration and Technique of Environmental Monitoring

基　　金：国家科技重大专项子课题“太湖流域浮游生物快速鉴定技术验证及应用转化”基金资助项目(2018ZX07208002-02)。

摘　　要：采用水蒸气蒸馏萃取法处理新鲜采摘的桂花,利用飞行时间质谱(GC-Q-TOF/MS)法定性分析β-紫罗兰酮,考察β-紫罗兰酮的碎裂方式,再用气相色谱-三重四级杆串联质谱(GC-MS/MS)多重反应监测(MRM)定量分析桂花中β-紫罗兰酮。该方法在2.00μg/L~100μg/L范围内线性良好,方法检出限为8.67 ng/L。用该方法连续2 d测定新鲜桂花,β-紫罗兰酮均值为5.55 mg/kg。β-ionone was extracted from fresh Osmanthus fragrans(Thunb.)Lour.by steam distillation,and was qualitatively analyzed by time of fight mass spectrometry(GC-Q-TOF/MS),quantitatively analyzed by gas chromatography-triple quadrupole tandem mass spectrometry(GC-MS/MS)through investigating its fragmentation manner.By optimizing the test conditions,the method had good linearity at 2.00μg/L~100μg/L of the target substance,the method detection limit was 8.67 ng/L.The method was applied in determining fresh Osmanthus fragrans(Thunb.)Lour.for 2 days,β-ionone was detected in two batches of samples,and the average test results was 5.55 mg/kg.

关 键 词：Β-紫罗兰酮 飞行时间质谱法 气相色谱-三重四级杆串联质谱法 桂花 

分 类 号：O657.63[理学—分析化学]