高效液相色谱法测定土壤中甲醛空白值影响因素探讨  

Influence Factor of Formaldehyde Blank Value in Soil by High Performance Liquid Chromatography

作　　者：熊杰[1,2] 杨菊 王若男[2] 何吉明 曹阳 李海霞 XIONG Jie;YANG Ju;WANG Ruo-nan;HE Ji-ming;CAO Yang;LI Hai-xia(Chengdu University of Technology,Chengdu,Sichuan 610059,China;Ecology and Environmental Monitoring of Sichuan,Chengdu,Sichuan 610091,China)

机构地区：[1]成都理工大学,四川成都610059 [2]四川省生态环境监测总站,四川成都610091

出　　处：《环境监测管理与技术》2022年第2期68-71,共4页The Administration and Technique of Environmental Monitoring

基　　金：国家重点研发计划“成渝地区大气污染联防联控技术与集成示范”基金资助项目(2018YFC0214004);四川省科技厅“成都市重污染过程PM_(2.5)污染特征及来源解析研究”基金资助项目(2018JY0518);四川省教育厅“成都市PM_(2.5)污染特征及主要气态前体物对其影响研究”基金资助项目(18ZB0064)。

摘　　要：采用高效液相色谱法测定土壤中甲醛,探讨试验前处理过程中使用的试剂、耗材、玻璃器皿的洗涤,以及萃取流量、液体在环境中暴露时长等操作细节对空白值的影响。试验表明:衍生剂2,4-二硝基苯肼须纯化后使用;提取液应使用超纯水机现制水配制,且现配现用;石英砂须经高温灼烧后放置在干燥器中保存;玻璃器皿应在使用前烘干或者再次用纯水冲洗。采用固相萃取进行萃取浓缩时,盛装衍生液的三角瓶瓶口应全程密封,萃取过程操作时间应控制在20 min~40 min之间,过柱后空抽时间应控制在1 min内。优化操作后,空白样品中甲醛值可低于20μg/L。In this paper,the effects of reagents,consumables,washing of glassware,extraction flow rate and exposure time of liquid in the environment on the blank value were discussed for the determination of formaldehyde in soil by HPLC.The results showed that the derivative agent of 2,4-dinitrophenylhydrazine should be purified before using.The extract solution should be prepared fresh by ultrapure water and ready to use.Quartz sand should be burned at high temperature and stored in a dryer.Glassware should be dried or rinsed with pure water again before use.When solid phase extraction was used for extraction and concentration,the mouth of the triangular bottle containing derivative solution should be sealed throughout the whole process,the operation time of extraction should be controlled within 20 min~40 min,and the vacuuming time should be controlled within 1 min after passing the column.After optimization operation,formaldehyde in blank sample could be lower than 20μg/L.

关 键 词：甲醛 空白值 高效液相色谱法 影响因素 土壤 

分 类 号：O657.72[理学—分析化学]