1株阿尔巴斯绒山羊源产气荚膜梭菌的毒素型鉴定和16S rRNA基因的遗传进化分析  

Toxin genotyping identification and genetic evolution analysis of 16S rRNA gene of a strain of Clostridium perfringens from Alpas cashmere goats

作　　者：李琦[1,2] 白伟琴 李杰 李宏 赵利军 孟克 訾占飞 格日勒图 吉林台[1,2] 王金玲 卡楚拉 LI Qi;BAI Weiqin;LI Jie;LI Hong;ZHAO Lijun;MENG Ke;ZI Zhanfei;Geriletu;Jilintai;WANG Jinling;Kachula(College of Veterinary Medicine,Inner Mongolia Agricultural University,Hohhot 010010,China;Key Laboratory of Clinical Diagnosis and Treatment Technology in Animal Disease,Ministry of Agriculture and Rural Affairs,Hohhot 010010,China;Ejin Horo Banner Center for Animal Disease Control and Prevention,Ordos 017200,China)

机构地区：[1]内蒙古农业大学兽医学院,呼和浩特010010 [2]农业农村部动物疾病临床诊疗技术重点实验室,呼和浩特010010 [3]伊金霍洛旗动物疫病预防控制中心,内蒙古鄂尔多斯017200

出　　处：《黑龙江畜牧兽医》2022年第6期68-71,76,共5页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(RZ1900003523)。

摘　　要：为了了解1例经诊断为产气荚膜梭菌病而死亡的阿尔巴斯种绒山羊感染的产气荚膜梭菌的毒素型和16S rRNA基因的遗传进化特性,试验设计5对特异性毒素引物,采用PCR方法进行毒素型的鉴定,对16S rRNA基因扩增和测序,对测序结果进行遗传进化分析。结果表明:只有α毒素引物扩增出约475 bp长的目的条带,阿尔巴斯绒山羊梭菌性肠炎的致病菌毒素为α毒素,为A型产气荚膜梭菌,其16S rRNA基因序列与天津地区产气荚膜梭菌(登录号为KP944160.1)位于同一分支,亲缘关系最近,核苷酸相似性为98.53%。说明此次阿尔巴斯绒山羊感染的产气荚膜梭菌的毒素型是A型,且鉴定菌株与天津地区产气荚膜梭菌(登录号为KP944160.1)起源于共同的祖先。In order to understand the toxin genotyping of Clostridium perfringens and the genetic evolution of 16 S rRNA gene in a case of Alpas cashmere goat who died after being diagnosed with Clostridium perfringens,five pairs of specific toxin primers were designed in the experiment,and the toxin type was identified by PCR method;the 16 S rRNA gene was amplified and sequenced,and the sequencing results were analyzed for genetic evolution.The results showed that only theα-toxin primer amplified the target band of about 475 bp,and the pathogenic bacterial toxin of Clostridium enteritis in Alpas cashmere goat wasα-toxin,which was Clostridium perfringens type A,whose 16 S rRNA gene sequence was located in the same branch as Clostridium perfringens in Tianjin area(Accession number KP944160.1),with the closest relationship,and the nucleotide similarity was 98.53%.The results suggested that the toxin type of Clostridium perfringens infected by Alpas cashmere goat was type A,and the identified strain and Clostridium perfringens in Tianjin area(Accession number KP944160.1)originated from the common ancestor.

关 键 词：阿尔巴斯绒山羊 产气荚膜梭菌 毒素型 16S rRNA基因 遗传进化 

分 类 号：S852.616.3[农业科学—基础兽医学]