基于网络药理学探讨连翘、金银花药对挥发油抗流感作用机制的研究  

Study on the mechanism of the anti-influenza role of Forsythia and Lonicera japonica on volatile oil based on network pharmacology

作　　者：韩愈杰 李定刚[2,3] 杨鹤云 任强[3] 赵兴华[1,2] HAN Yujie;LI Dinggang;YANG Heyun;REN Qiang;ZHAO Xinghua(College of Traditional Chinese Veterinary Medicine,Hebei Agricultural University,Baoding 071000,China;Hebei Province Research Center of Traditional Veterinary Medicine Engineering Technology,Baoding 071100,China;Baoding Jizhong Pharmaceutical Co.,Ltd.,Baoding 071500,China)

机构地区：[1]河北农业大学中兽医学院,河北保定071000 [2]河北省中兽药工程技术研究中心,河北保定071100 [3]保定冀中药业有限公司,河北保定071500

出　　处：《黑龙江畜牧兽医》2022年第6期120-124,140,141,共7页Heilongjiang Animal Science And veterinary Medicine

摘　　要：为了探讨连翘、金银花药对挥发油抗流感的作用机制,试验采用网络药理学方法,通过文献检索法查询连翘、金银花挥发油的化学成分,并通过Swiss Target Prediction数据库获得其作用靶点,在DisGeNET数据库搜索流感相关靶点,于Venny 2.1在线作图工具平台获得挥发油与流感交集靶点,运用Cytoscape 3.8软件构建挥发油活性成分-抗流感靶点网络图。利用STRING数据库构建蛋白质互作网络图,通过拓扑参数筛选得到治疗流感的核心靶点,再进行GO富集和KEGG信号通路分析。结果表明:筛选得到连翘、金银花药挥发油30个活性成分及与作用流感病毒相关的59个靶点,其中核心靶点为IL-6、HSP90AA1、CYP2C19及CCR5。核心靶点参与对外源物质的反应、激素水平的调节、细胞对脂质的反应及对铵离子的反应等多种生物学过程,作用于神经活性配体受体信号通路、5-羟色胺突触信号通路、cAMP信号通路及细胞因子相互作用信号通路。说明连翘、金银花药对挥发油治疗流感涉及多成分、多靶点、多通路的机制。In order to explore the mechanism of Forsythia and Lonicera japonica on the anti-influenza volatile oil, the network pharmacology method was used in the experiment.The chemical constituents of the volatile oil of Forsythia and Lonicera japonica were searched by literature retrieval;their targets were obtained through the Swiss Target Prediction database;the influenza-related targets were searched in the DisGeNET database;and the intersection targets of volatile oil and influenza were obtained on the Venny 2.1 online mapping tool platform;the volatile oil active component-anti-influenza target network map was constructed using Cytoscape 3.8 software.The STRING database was used to construct a protein interaction network map, and the core targets for the treatment of influenza were screened by topological parameters, and then GO enrichment and KEGG signaling pathway analysis were performed. The results showed that 30 active components of Forsythia and Lonicera japonica volatile oil and 59 targets related to influenza were screened, among which the core targets were IL-6, HSP90 AA1, CYP2 C19 and CCR5. GO enrichment and KEGG signal pathway analysis showed that the core targets were involved in various biological processes, such as the response to exogenous substances, the regulation of hormone levels, the response of cells to lipids, and the response to ammonium ions, acting on neuroactive ligand receptor signaling pathway, 5-serotonin synaptic signaling pathway, cAMP signaling pathway and cytokine interaction signaling pathway. The results suggested that the mechanism of multi-components, multi-targets and multi-pathways were involved in the treatment of influenza with volatile oil of Forsythia and Lonicera japonica.

关 键 词：连翘金银花油 流感 网络药理学 相互作用 作用机制 

分 类 号：S859.7[农业科学—临床兽医学]