喉鳞状细胞癌组织中朊蛋白分子特征的分析  

Prion protein molecular characteristics in laryngeal squamous cell carcinoma

作　　者：魏炜[1] 武月章 陈冬冬 宋韫韬[1] 徐国辉[1] 石琦[2] Wei Wei;Wu Yuezhang;Chen Dongdong;Song Yuntao;Xu Guohui;Shi Qi(Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education),Head and Neck Surgery Department,Peking University Cancer Hospital&Institute,Beijing 100142,China;State Key Laboratory for Infectious Disease Prevention and Control,National Institute for Viral Disease Control and Prevention,Chinese Center for Disease Control and Prevention,Beijing 102206,China)

机构地区：[1]北京大学肿瘤医院暨北京市肿瘤防治研究所头颈外科恶性肿瘤发病机制及转化研究教育部重点实验室,北京100142 [2]中国疾病预防控制中心病毒病预防控制所,传染病预防控制国家重点实验室,北京102206

出　　处：《疾病监测》2022年第2期176-179,共4页Disease Surveillance

基　　金：传染病预防控制国家重点实验室课题(No.2019SKLID501)。

摘　　要：目的 观察喉鳞状细胞癌组织中朊蛋白的分子特征。方法 采用免疫印迹法检测朊蛋白的带型特征,采用免疫组织化学法检测朊蛋白在组织细胞中的分布特征,采用基因测序分析的方法检测分析朊蛋白基因特征。结果 免疫印迹的方法显示,喉鳞状细胞癌组织中,朊蛋白呈现3种糖基化形式;免疫组织化学法显示,朊蛋白在喉鳞状细胞癌中可出现阳性表达,主要分布在细胞膜及胞浆。随着肿瘤恶性程度的增加,朊蛋白的表达呈逐渐增加的趋势;基因测序显示,纳入样本朊蛋白均无突变,朊蛋白129位密码子均为甲硫氨酸纯合子,219位均为谷氨酸纯合子。结论 喉鳞状细胞癌组织中,朊蛋白特征有别于中枢神经系统,如脑组织;其表达与肿瘤的恶性程度相关,有可能成为早期分析诊断喉鳞状细胞的分子靶标。Objective To observe the molecular characteristics of prion protein in laryngeal squamous cell carcinoma tissues. Methods Molecular characteristics of prion protein in laryngeal squamous cell carcinoma tissues were analyzed with the help of western blot. Distribution characteristics of prion protein and PRNP genetic features in laryngeal squamous cell carcinoma tissue were analyzed by the methods of immunohistochemical and gene sequencing assay, respectively. Results Western blots showed that three types of glycosylation forms of prion proteins were presented in laryngeal squamous cell carcinoma tissues. Immunohistochemical assay showed positive expression of prion protein and mainly distributed in cell membrane and cytoplasm, with the increasing of the malignancy in tumor, the level of prion protein increases. Gene sequencing illustrated no mutation in PRNP gene. All codon 129 of PRNP gene were homozygous for methionine and that of 219 were homozygous for glutamate. Conclusion Characteristics of prion protein in laryngeal squamous cell carcinoma tissues are different from center nerve system like brain tissue. Its expression levels were associated with the malignant degree of tumor, which is possible to be a molecular marker for early diagnosis of laryngeal squamous cell carcinoma.

关 键 词：喉鳞状细胞癌 朊蛋白 分子特征 

分 类 号：R211[医药卫生—中医学] R511