黄芪葛根汤对高脂血症大鼠脂质代谢和氧化应激的影响  

Impact of Huangqi Gegen Formula on Lipid Metabolism and Oxidative Stress in Hyperlipidemia Rats

作　　者：周遵明 谭梅傲 彭冲 于小庆[1] 叶晋通 凡思敏 柯雪红 黄可儿[1] ZHOU Zun-ming;TAN Mei-ao;PENG Chong;YU Xiao-qing;YE Jin-tong;FAN Simin;KE Xue-hong;HUANG Ke-er(Department of Gastroenterology,The First Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou,510405;The First Clinical Medical College of Guangzhou University of Chinese Medicine,Guangzhou,510405;Lingnan Medical Research Center,Guangzhou University of Chinese Medicine,Guangzhou,510405)

机构地区：[1]广州中医药大学第一附属医院脾胃科,广州510405 [2]广州中医药大学第一临床医学院,广州510405 [3]广州中医药大学岭南医学研究中心,广州510405

出　　处：《中国中西医结合杂志》2022年第2期195-201,共7页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：广州中医药大学学科研究重点项目(No.XK2019011);广州中医药大学第一附属医院“创新强院科研项目之院内制剂开发项目”(No.211010010602);国家自然科学基金项目(No.82074099)。

摘　　要：目的观察黄芪葛根汤对高脂饲料(HFD)诱导的高脂血症模型大鼠的影响。方法将50只大鼠随机分为正常组、模型组、阿托伐他汀钙组(阿托伐他汀钙片5 mg/kg)、黄芪葛根汤低剂量组(5 g/kg)、黄芪葛根汤中剂量组(10 g/kg),每组10只;采用HFD喂养制备高脂血症模型大鼠,造模同时灌胃给药,正常组和模型组给予等量生理盐水,每天1次,连续灌胃20天。采用全自动生化分析仪检测血清TC、TG、HDL-C和LDL-C水平,HE染色及油红染色观察大鼠肝脏病理变化,ELISA法测定肝组织活性氧簇(ROS)、超氧化物歧化酶(SOD)、丙二醛(MDA)及谷胱甘肽过氧化物酶(GSH)水平,RT-PCR及Western Blot法检测核转录因子NF-E2相关因子2(Nrf2)和血红素加氧酶-1(HO-1)mRNA及蛋白的表达水平。结果与正常组比较,模型组血清TC、TG、LDL-C和肝脏ROS、MDA水平升高(P<0.01),血清HDL-C、肝脏Nrf2、HO-1 mRNA及蛋白和肝脏GSH、SOD水平下降(P<0.01,P<0.05),肝脏脂肪、脂滴显著堆积。与模型组比较,黄芪葛根汤低剂量组血清TC、TG、LDL-C和肝脏ROS、MDA水平降低(P<0.01,P<0.05),Nrf2、HO-1 mRNA和蛋白水平及肝脏GSH、SOD水平升高(P<0.01,P<0.05),模型大鼠肝脏脂肪堆积和脂滴积聚改善。结论黄芪葛根汤对HFD诱导的高脂血症大鼠具有降血脂作用,并通过激活Nrf2/HO-1通路改善高脂血症大鼠的氧化应激状态。Objective To observe the effect of Huangqi Gegen Formula(HGF)on hyperlipidemia rats induced by high fat diet(HFD).Methods Fifty rats were randomly divided into normal group,model group,atorvastatin calcium group(atorvastatin calcium tablet 5 mg/kg),low-dose HGF group(5 g/kg)and medium-dose HGF group(10 g/kg),10 in each group.Hyperlipidemia model rats were prepared by feeding HFD,and were given intragastric administration at the same time.Normal group and model group were given the equal volume of normal saline once a day for 20 consecutive days.Serum TC,TG,HDL-C and LDL-C levels were detected by automatic biochemical analyzer,and the pathological changes of liver were observed by HE staining and Oil red staining.The levels of hepatic reactive oxygen species(ROS),superoxide dismutase(SOD),malondialdehyde(MDA)and glutathione peroxidase(GSH)were determined by ELISA.RT-PCR and Western Blot were used to detect the mRNA and protein expressions of hepatic nuclear factor erythroid-2-related factor 2(Nrf2)and heme oxygenase-1(HO-1).Results Compared with the normal group,serum TC,TG and LDL-C,as well as hepatic ROS and MDA levels increased significantly(P<0.01),serum HDL-C,hepatic Nrf2 and HO-1 mRNA and protein expression,as well as hepatic GSH and SOD levels decreased significantly(P<0.01,P<0.05).Hepatic fat and lipid droplets accumulated significantly in the model group.Compared with the model group,serum TC,TG and LDL-C,as well as hepatic ROS and MDA levels decreased significantly(P<0.01,P<0.05),Nrf2,HO-1 mRNA and protein expression,as well as hepatic GSH and SOD levels increased significantly(P<0.01,P<0.05)in low-dose HGF group.The accumulation of fat and lipid droplets in model rats were obviously improved in lowdose HGF group.Conclusion HGF can reduce blood lipid levels in hyperlipidemia rats induced by HFD,and improve the oxidative stress of hyperlipidemia rats by activating Nrf2/HO-1 pathway.

关 键 词：高脂血症 黄芪葛根汤 氧化应激 Nrf2/HO-1通路 

分 类 号：R285.5[医药卫生—中药学]