癸酸钠抗性菌株产达托霉素的发酵补料策略  

Fermentation Feed Strategy for Daptomycin Production by Sodium Caprate-resistant Strains

作　　者：顾晶 石彦鹏 牛春 张萍 GU Jing;SHI Yan-peng;NIU Chun;ZHANG Ping(Ningxiatairui Pharmaceutical Limited Company,Yinchuan 750101,China)

机构地区：[1]宁夏泰瑞制药股份有限公司,宁夏银川750101

出　　处：《黑龙江农业科学》2022年第4期81-84,共4页Heilongjiang Agricultural Sciences

摘　　要：为提高达托霉素产生菌玫瑰孢链霉菌的发酵能力,通过筛选癸酸钠抗性菌株,优化其发酵瓶补加正癸酸的方法,提高达托霉素的摇瓶效价值。首先对原始菌株进行癸酸钠抗性选育,筛选出一株空白摇瓶效价值为43.4 mg·L^(-1)的菌株TR21-29,利用该菌株对癸酸盐的耐受性,验证其发酵瓶补加正癸酸的最优方式。主要考察正癸酸的耦合剂,向发酵瓶添加正癸酸的补料时间、补料浓度和补料间隔,确定发酵过程中补加正癸酸的关键参数。结果表明:补料可将抗性菌株TR21-29的原始效价值由43.4 mg·L^(-1)提高至138.1 mg·L^(-1),提高率为218.2%。补料方式为配制0.5 g·mL^(-1)的正癸酸溶液,在发酵瓶培养24 h后开始补加,补料终浓度为1.5 mmol·L^(-1),补料间隔为4 h。In order to improve the fermentation ability of daptomycin-producing bacterium streptomyces roseolae,by screening sodium caprate resistant strains,optimizing the method of supplementing N-decanoic acid in its fermentation flask,improving the shake flask effect value of tropicolin.The original strain was selected for sodium caprate resistance,a strain TR21-29 with a blank shake flask efficacy value of 43.4 mg·L^(-1) was screened out.We took advantage of the strain′s tolerance to caprate,verified the optimal strategy of supplementing N-decanoic acid in its fermentation flask.This study mainly investigated the coupling agent of N-decanoic acid,and feed time,feed concentration and feed interval for adding N-decanoic acid to fermentation flasks,determining the key parameters for the addition of N-decanoic acid to fermentation flasks.The results showed that the original efficacy value of the resistant strain TR21-29 was increased from 43.4 mg·L^(-1) to 138.1 mg·L^(-1),and the improvement rate was 218.2%.The feeding method is to start adding N-decanoic acid solution after culturing in the fermentation flask for 24 hours.The concentration of N-decanoic acid is 0.5 g·L^(-1),the feed final concentration is 1.5 mmol·L^(-1),and the feed is fed every 4 hours.

关 键 词：达托霉素 玫瑰孢链霉菌 癸酸钠 正癸酸 发酵瓶补料 

分 类 号：TS2[轻工技术与工程—食品科学与工程]