摘花对不同种源不同生长时期浙贝母不同部位中3种生物碱含量的影响  

Effect of Flower Removal on Content of Three Alkaloids in Different Parts of Fritillaria thunbergii from Different Regions and at Different Growth Stages

作　　者：王丹 李金金 杨青山 许凌峰 周游 周浓 WANG Dan;LI Jin-jin;YANG Qing-shan;XU Ling-feng;ZHOU You;ZHOU Nong(Chongqing Engineering Laboratory for Green Planting and Deep Processing of Genuine Medical Materials in Three Gorges Reservoir Area,College of Biology and Food Engineering,Chongqing Three Gorges University,Chongqing 404120,China)

机构地区：[1]重庆三峡学院生物与食品工程学院,三峡库区道地药材绿色种植与深加工重庆市工程实验室,重庆404120

出　　处：《中国实验方剂学杂志》2022年第8期159-166,共8页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：重庆市技术创新与应用示范项目(cstc2018jscx-msyb X0367)。

摘　　要：目的:研究摘花对不同种源浙贝母在不同生长时期的不同部位中3种生物碱含量的影响。方法:采用超高效液相色谱-蒸发光散射(UPLC-ELSD)法,ACQUITY UPLC BEH C;色谱柱(2.1 mm×150 mm,1.7μm);流动相0.02%三乙胺水溶液(A)和甲醇(B)梯度洗脱(0~2 min,45%A;2~5 min,45%~25%A;5~7 min,25%A;7~17 min,25%~10%A;17~20 min,10%A),流速0.20 m L·min^(-1),柱温35℃,样品室温度20℃,漂移管温度45℃;喷雾器参数40%,进样量为3μL,同时测定不同生长时期不同种源摘花处理及未摘花的浙贝母鳞茎、须根、茎和叶中贝母素甲、贝母素乙和贝母辛的含量。结果:摘花处理可以明显提高不同种源浙贝母的产量(P<0.05);现蕾期种源为浙江宁波、浙江磐安和江苏南通的浙贝母摘花处理后鳞茎中生物碱含量较未摘花有明显提高(P<0.05),种源为重庆奉节的浙贝母摘花与未摘花处理鳞茎中生物碱含量差异无统计学意义;花期种源为江苏南通的浙贝母摘花处理后鳞茎中生物碱含量明显高于未摘花处理(P<0.05),种源为浙江磐安和重庆奉节的浙贝母摘花后鳞茎中生物碱含量明显低于未摘花处理(P<0.05),种源为浙江宁波的浙贝母摘花与未摘花处理鳞茎中生物碱含量差异无统计学意义;鳞茎膨大期种源为浙江宁波和浙江磐安的浙贝母摘花处理后鳞茎中生物碱含量明显高于未摘花处理(P<0.05),种源为江苏南通的浙贝母摘花后鳞茎中生物碱含量明显低于未摘花处理(P<0.05),种源为重庆奉节的浙贝母摘花与未摘花处理鳞茎中生物碱含量差异无统计学意义;收获期除种源为浙江磐安的浙贝母摘花处理后鳞茎中生物碱含量明显高于未摘花处理外(P<0.05),其余种源的浙贝母摘花后鳞茎中生物碱含量均明显低于未摘花处理(P<0.05);各处理的不同种源浙贝母叶中生物碱含量在各生长时期均明显高于鳞茎(P<0.05)。结论:人工摘花处理可以提高浙贝母产量,摘花后多数浙�Objective:To study the effect of flower removal on the content of three alkaloids in different parts of Fritillaria thunbergii from different regions and at different growth stages.Method:The content of peiminine,peimine,and peimisine in the bulb,root,stem,and leaf of F.thunbergii after flower removal and with flower un-removed at different growth stages and in different regions were determined simultaneously by ultra-performance liquid chromatography-evaporative light scattering detection(UPLC-ELSD)method.The UPLC was conducted on ACQUITY UPLC BEH C;column(2.1 mm×150 mm,1.7μm)with the mobile phase of 0.02%triethylamine aqueous solution(A)and methanol(B)elution gradient(0-2 min,45%A;2-5 min,45%-25%A;5-7 min,25%A;7-17 min,25%-10%A;17-20 min,10%A),flow velocity of0.20 m L·min^(-1),column temperature 35°C,sample room temperature of 20°C,and injection volume of 3μL.The ELSD was carried out at drift tube temperature 45°C and with the sprayer parameter of 40%.Result:The flower removal significantly increased the yield of F.thunbergii.At the budding stage,the alkaloid content in the bulb of F.thunbergii from Ningbo in Zhejiang,Pan’an in Zhejiang,and Nantong in Jiangsu after flower removal were significantly higher than that of flowering un-removal treatment,while it showed no significant difference between the flower removal and un-removal treatments for the samples from Fengjie in Chongqing.At the flowering stage,the alkaloid content in the bulb of F.thunbergii from Nantong in Jiangsu after flower removal was significantly higher than that of flower un-removal treatment,while it showed an opposite trend for the samples from Pan’an in Zhejiang and Fengjie in Chongqing and had no significant difference between the two treatments for the samples from Ningbo in Zhejiang.At the bulb expansion stage,the alkaloid content in the bulb of F.thunbergii from Ningbo in Zhejiang and Pan’an in Zhejiang after flower removal were significantly higher than that of flower un-removal treatment,which was opposite for the sampl

关 键 词：浙贝母 种源 摘花 生物碱含量 超高效液相色谱-蒸发光散射(UPLC-ELSD) 不同生长时期 

分 类 号：R284.2[医药卫生—中药学] R289[医药卫生—中医学]