Desmoglein 2(DSG2) Is A Receptor of Human Adenovirus Type 55 Causing Adult Severe Community-Acquired Pneumonia  

作　　者：Jing Zhang Kui Ma Xiangyu Wang Yinbo Jiang Shan Zhao Junxian Ou Wendong Lan Wenyi Guan Xiaowei Wu Heping Zheng Bin Yang Chengsong Wan Wei Zhao Jianguo Wu Qiwei Zhang 

机构地区：[1]Guangdong Provincial Key Laboratory of Virology,Institute of Medical Microbiology,Jinan University,Guangzhou,510632,China [2]Guangdong Provincial Key Laboratory of Tropical Disease Research,School of Public Health,Southern Medical University,Guangzhou,510515,China [3]Guangdong Provincial Dermatology Hospital,Southern Medical University,Guangzhou,510091,China

出　　处：《Virologica Sinica》2021年第6期1400-1410,共11页中国病毒学（英文版）

基　　金：This work was supported by grants from the National Key Research and Development Program of China(2018YFE0204503);Natural Science Foundation of Guangdong Province(2021A1515010788 and 2018B030312010);the Guangzhou Healthcare Collaborative Innovation Major Project(201803040004 and 201803040007)。

摘　　要：Human adenovirus type 55(HAdV-B55) is a re-emergent acute respiratory disease pathogen that causes adult communityacquired pneumonia(CAP). Previous studies have shown that the receptor of HAdV-B14, which genome is highly similar with HAdV-B55, is human Desmoglein 2(DSG2). However, whether the receptor of HAdV-B55 is DSG2 is undetermined because there are three amino acid mutations in the fiber gene between HAdV-B14 and HAdV-B55. Here, firstly we found the 3T3 cells, a mouse embryo fibroblast rodent cell line which does not express human DSG2, were able to be infected by HAdV-B55 after transfected with pcDNA3.1-DSG2, while normal 3T3 cells were still unsusceptible to HAdV-B55 infection. Next, A549 cells with h DSG2 knock-down by siRNA were hard to be infected by HAdV-B3/-B14/-B55, while the control siRNA group was still able to be infected by all these types of HAdVs. Finally, immunofluorescence confocal microscopy indicated visually that Cy3-conjugated HAdV-B55 viruses entered A549 cells by binding to DSG2 protein.Therefore, DSG2 is a major receptor of HAdV-B55 causing adult CAP. Our finding is important for better understanding of interactions between adenoviruses and host cells and may shed light on the development of new drugs that can interfere with these processes as well as for the development of potent prophylactic vaccines.

关 键 词：Human adenovirus type 55(HAdV-B55) Severe community-acquired pneumonia Adenovirus receptor Desmoglein 2(DSG2) 

分 类 号：R373[医药卫生—病原生物学]