Generation and Application of a Luciferase Reporter Virus Based on Yellow Fever Virus 17D  

作　　者：Dan Li Jing-Long Ye Zhong-Yu Liu 

机构地区：[1]The Centre for Infection and Immunity Studies,School of Medicine,Sun Yat-Sen University,Guangzhou 510006,China

出　　处：《Virologica Sinica》2021年第6期1456-1464,共9页中国病毒学（英文版）

基　　金：This work is supported by the National Natural Science Foundation of China(81871632 and 32070183);the Natural Science Foundation of Guangdong Province(2020A1515010656);the Creative Research Group Foster Project of the Sun Yat-sen University;supported by the One-Hundred People Project of the Sun Yat-sen University。

摘　　要：Yellow fever virus(YFV) is a re-emerging virus that can cause life-threatening yellow fever disease in humans.Despite the availability of an effective vaccine,little is known about the replication mechanism of YFV,and there are still no available specific anti-YFV medicines.Herein,by introducing the Renilla luciferase gene(Rluc) into an infectious clone of YFV vaccine strain 17 D,we generated a recombinant virus 17 D-Rluc.2 A via reverse genetics approaches.The 17 D-Rluc.2 A had similar plaque morphology and comparable in vitro growth characteristics with its parental strain.Importantly,the reporter luciferase was efficiently expressed in 17 D-Rluc.2 A-infected mammalian and mosquito cells,and there was a good linear correlation between intracellular luciferase expression and extracellular infectious virion reproduction.Furthermore,by a combination of the 17 D-Rluc.2 A reporter virus and selective 2’-hydroxyl acylation analyzed by primer extension(SHAPE)technology,the conserved 5’-SLA element was shown to be essential for YFV replication,highlighting the capability of17 D-R1 uc.2 A in the investigation of YFV replication.At last,we demonstrated that two compounds with distinct anti-viral mechanisms can effectively inhibit the viral propagation in 17 D-Rluc.2 A-infected cells,demonstrating its potential application in the evaluation of anti-viral medicines.Taken together,the 17 D-Rluc.2 A serves as a useful tool for the study of YFV replication and anti-YFV medicine development.

关 键 词：Yellow fever virus(YFV) 17D Reporter virus Cis-acting RNA elements Anti-viral medicines 

分 类 号：R373[医药卫生—病原生物学]