Photofrin联合碘化钾抗白色念珠菌光动力效果的研究  

Study on Photodynamic Effect and Mechanism of Photofrin plus Potassium Lodide on Candida Albicans

作　　者：杨善林 蓝丽娜 黄力毅[1] 宣伟军[3] 何丽霞[1] 柒泳奇 甑秀梅 YANG Shanlin;LAN Lina;HUANG Liyi;XUAN Weijun;HE Lixia;QI Yongqi;ZENG Xiumei(Department of Infectious Diseases,First Affiliated Hospital of Guangxi Medical University,Nanning 530021,China;AIDS Research Center of Guangxi Medical University;Chinese Medicine University of Guangxi)

机构地区：[1]广西医科大学第一附属医院感染性疾病科,南宁市530021 [2]广西医科大学艾滋病研究中心 [3]广西中医药大学

出　　处：《中国激光医学杂志》2022年第1期6-12,58,59,共9页Chinese Journal of Laser Medicine & Surgery

基　　金：国家自然科学基金(81860369,81472002);广西自然科学基金面上项目(2016GXNS-FAA380312)。

摘　　要：目的探讨光敏剂(photosensitizer,PS)Photofrin(PF)联合不同浓度碘化钾(potassium iodide,KI)介导的抗菌光动力疗法(antimicrobial photodynamic therapy,aPDT)抗白色念珠菌(Candida albicans,C.albicans)的效果。方法实验对象C.albicans(DAY286),进行三部分实验分别为:(1)PF及PF联合KI介导的aPDT对C.albicans的杀伤作用。实验分为PDT组(A组)和对照组(B组),A组处理分别为:(1)PF-PDT,取10^(7)CFU/ml的C.albicans菌悬液1 ml与PF(终浓度分别为0.1、0.2、1、2和4μM)1 ml混匀,室温避光孵育30 min,行能量密度10 J/cm^(2)的蓝光光照;(2)PF+KI-PDT,取菌悬液1 ml与PF(终浓度分别为5、10μM)1 ml混匀,室温避光孵育30 min,再添加KI(终浓度分别为0、1、10、25、50和100 mM)1 ml后,行能量密度10 J/cm^(2)的蓝光光照。B组处理分别为:(1)仅加入PF或仅加入PF+KI,光照或不光照;(2)仅加入菌悬液,不加入PF、KI也不光照。(2)单线态氧猝灭剂叠氮化钠(sodium azide,NaN_(3))对PF+KI-aPDT抗菌作用的影响。实验分为aPDT组(A组)和空白对照组(B组)。A组首先将107CFU/ml的C.albicans菌悬液1 ml与PF(终浓度为10μM)1 ml混匀后避光孵育30 min,然后分为4个小组:(1)不加入KI、NaN_(3),仅光照;(2)加入KI(终浓度为100 mM)后光照;(3)加入NaN_(3)(终浓度为50 mM)后光照;(4)加入KI、NaN_(3)后光照,4小组均采用能量密度10 J/cm^(2)光照。B组:空白对照组,仅接种菌悬液,不加入PF、KI、NaN_(3),也不光照。(3)两种荧光探针检测PF光化学特性。实验分为单线态氧荧光探针(singlet oxygen sensor green reagent,SOSG)组(A组)、羟基自由基荧光探针[3’-(p-hydroxyphenyl)-fluorescein,HPF]组(A0组)和对照组(B组)。A组先将SOSG(终浓度为10μM)与PF(终浓度为10μM)混匀,加或不加入KI(终浓度为100 mM),构成总体积200μl,光照;A0组将HPF(终浓度为10μM)与PF混匀,加入或不加入KI,光照;B1、B2组:不加PF及KI,分别加入SOSG和HPF,光照;B3、B4组:分别加入SOSG和HPF,两组均加入KI,不加PF,�Objective To explore the antimicrobial photodynamic therapy(aPDT)effect of photofrin(PF)combined with different concentrations of potassium iodide(KI)on Candida albicans(C.albicans)Methods C..albicans standard strain(DAY286)was prepared as the experimental subject.The experiment was divided into three parts.Part one:the PF-aPDT and PF+KI-aPDT killing effect on C.albicans.In this part,two groups were established,namely,the PDT group(group A)and control group(group B).The treatments for group A were as follows:firstly,1 ml of C.albicans suspension(10^(7) CFU/mL)was mixed with 1 ml of PF(final concentration:0.1,0.2,1,2 and 4μM),and incubated at room temperature for 30 min then irradiated with blue light of 10 J/cm^(2);secondly,1 ml of bacterial suspension was mixed with 1 ml of PF(final concentration:5 and 10μM),and incubated at room temperature for 30 min without light.The suspension was then exposed to 10 J/cm^(2)of blue light upon the addition of 1 ml of KI(final concentration:0,1,10,25,50 and 100 mM).The treatment for Group B was as follows:firstly,the suspension was mixed with PF with or without KI,and then exposed to light or not;secondly,neither PF nor light were given to the C.albicans suspension.Part two:the effect of sodium azide(NaN_(3))on the killing effect of PF+KI-aPDT.In this part of experiment,two groups were formed,i.e.,the aPDT group(group A)and blank control group(group B).For group A,1 ml of C.albicans suspension(107 CFU/ml)was mixed with 1 ml of PF(final concentration:10μM),then incubated for 30 min without light.The suspension was further divided into four groups.For the first group,neither KI nor NaN_(3)was added except of the illumination of10 J/cm^(2)blue light.For the second group,the suspension was illuminated after being added KI(final concentration:100 mM).For the third group,upon the addition of NaN_(3)(final concentration:50 mM),the suspension was illuminated with light.For the fourth group,the suspension was added with KI and NaN_(3)and then illuminated with light.For the blank con

关 键 词：抗菌 光动力疗法 碘化钾 白色念珠菌 光化学反应 

分 类 号：R454.2[医药卫生—治疗学] R379.4[医药卫生—临床医学]