钩藤中甾醇类化学成分研究  

Steroids from Uncaria rhynchophylla

作　　者：刘新宇 侯杏子 郭强 屠鹏飞 张庆英 LIU Xin-yu;HOU Xing-zi;GUO Qiang;TU Peng-fei;ZHANG Qing-ying(State Key Laboratory of Natural and Biomimetic Drugs and Department of Natural Medicines,School of Pharmaceutical Sciences,Peking University Health Science Center,Beijing 100191,China)

机构地区：[1]北京大学医学部药学院天然药物及仿生药物国家重点实验室和天然药物学系,北京100191

出　　处：《中国中药杂志》2022年第3期684-691,共8页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金项目(81872978)。

摘　　要：运用硅胶、ODS反相硅胶、Sephadex LH-20柱色谱以及制备高效液相色谱等多种方法对钩藤非生物碱部位的甾醇类成分进行了系统纯化分离,并采用NMR、MS等波谱方法鉴定其化学结构。从钩藤非生物碱部位中分离鉴定了13个甾醇类化合物(1~13),所有化合物均为首次从该属植物中分离鉴定,其中化合物1为新化合物,并首次对2个化合物(12、13)在氘代氯仿中的^(1)H-NMR和^(13)C-NMR数据进行了全归属。该研究丰富了钩藤化学成分的研究内容,提升了对钩藤中甾醇类成分的认知,为阐明钩藤药效物质基础以及钩藤的进一步开发和利用提供了参考依据。Thirteen steroids(1-13) were isolated from the non-alkaloid constituents of Uncaria rhynchophylla by column chromatography on silica gel,ODS,Sephadex LH-20,and preparative HPLC chromatography,and their structures were elucidated by analyses of the MS and NMR spectral data.All the compounds were isolated from the genus Uncaria for the first time,and 1 was a new compound.The^(1)H-NMR and^(13)C-NMR data of two compounds(12 and 13) in deuteron-chloroform were completely assigned.This study enriched the steroid constituents of U.rhynchophylla and provided scientific references for the elucidation of active constituents and further development and utilization of U.rhynchophylla.

关 键 词：钩藤 化学成分 甾醇 

分 类 号：R284.1[医药卫生—中药学]