p38MAPK介导的TGF-β1与BMP-7在泡型肝包虫病纤维化中的表达  

Expression of P38 MAPK-mediated TGF-β1 and BMP-7 in fibrosis of vesicular liver echinococcosis

作　　者：李栋 朱海宏[2] 陈敏 杨效 LI Dong;ZHU Haihong;CHEN Min;YANG Xiao(Chengdu BOE Hospital,Chengdu 610200,China;Qinghai Provincial People′s Hospital,Xining 810000,China)

机构地区：[1]成都京东方医院,四川成都610200 [2]青海省人民医院,青海西宁810000

出　　处：《西部医学》2022年第4期525-529,共5页Medical Journal of West China

基　　金：青海省卫计委重点课题项目基金资助(2018-wjzd-01)。

摘　　要：目的探讨p38、TGF-β1、BMP-7在泡型肝包虫病患者中不同纤维化程度肝组织中的基因表达差异及意义。方法收集2017年9月~2018年11月青海省人民医院包虫病区确诊的40位泡型肝包虫病患者术后切除肝组织,采集部位为距病灶边缘、距病灶2 cm内、距病灶>2 cm的肝脏组织。根据HE染色及Masson染色结果,按照knodell肝纤维化评分标准,将不同部位的标本纤维化分级,通过RT-PCR检测p38、TGF-β1和BMP-7在各级纤维化组中的表达。结果TGF-β1在S1、S2及S3组中相对表达量比较差异具有统计学意义(P<0.05)。p38在S1、S2及S3组中相对表达量比较差异具有统计学意义(P<0.05)。BMP-7在S、S2及S3组中相对表达量比较差异具有统计学意义(P<0.05)。p38与TGF-β1的相对表达量呈正相关(r=0.566,P<0.01)。BMP-7与TGF-β1的相对表达量呈负相关(r=-0.349,P<0.05)。结论TGF-β1可通过p38MAPK介导通路转导TGF-β1生物信号而发挥促纤维化生物效应;BMP-7在泡型肝包虫纤维化肝组织中异常表达与TGF-β1之间可能存在某种动态平衡关系,表达失衡可能加剧泡肝纤维化的演化发展。Objective To investigate the gene expression of P38,TGF-β1 and BMP-7 in liver tissues of patients with vesicular liver echinococcosis and their significance.Methods From September 2017 to November 2018,40 patients with vesicular hepatic echinococcosis diagnosed in the echinococcosis area of Qinghai Provincial People’s Hospital were collected for postoperative liver tissue resection.The collection sites were liver tissue from the edge of the lesion,within 2 cm from the lesion,and>2 cm from the lesion.According to HE staining and Masson staining results,according to Knodell liver fibrosis scoring standard,the fibrosis of samples in different parts were graded,and the expressions of P38,TGF-β1 and BMP-7 in different fibrosis groups were determined by RT-PCR.Results The relative expression levels of TGF-β1 in S1,S2 and S3 groups were(6.74±2.36),(8.97±2.60)and(11.57±2.22),respectively,with statistical significance(P<0.05).The relative expression levels of P38 in S1,S2 and S3 groups were(3.92±1.80),(6.51±3.16)and(9.26±2.84),respectively,with statistical significance(P<0.05).The relative expression levels of BMP-7 in S,S2 and S3 groups were(5.58±1.52),(8.79±2.41)and(3.91±1.59),respectively,and the difference was statistically significant(P<0.05).The relative expression level of TGF-β1 was positively correlated with P38(r=0.566,P<0.01).The relative expression level of TGF-β1 was negatively correlated with BMP-7(r=-0.349 P<0.05).Conclusion TGF-β1 can promote liver fibrosis by transduction of TGF-β1 biological signal through p38MAPK mediated pathway.There may be a dynamic equilibrium relationship between the abnormal expression of BMP-7 and TGF-β1 in the liver tissue of vesicular liver hydatid fibrosis,and the imbalance of expression may aggravate the evolution and development of vesicular liver fibrosis.

关 键 词：泡型肝包虫病 肝纤维化 转化生长因子Β1 人骨形态发生蛋白7 

分 类 号：R532.32[医药卫生—内科学] R575[医药卫生—临床医学]