DNA原位自组装用于细胞荧光成像分析研究  

Progress on In-situ DNA Self-assemblies for Cellular Fluorescence Imaging Analysis

作　　者：王思成 韩挺 王广凤[1] WANG Si-cheng;HAN Ting;WANG Guang-feng(Key Laboratory of Chem-Biosensing and Key Laboratory of Functional Molecular Solids of Anhui Province,College of Chemistry and Materials Science,Anhui Normal University,Wuhu 241000,China;Department of Chemistry,University of North Texas,Denton 76201,America)

机构地区：[1]安徽师范大学化学与材料科学学院,化学生物传感重点实验室和功能分子固体安徽省重点实验室,安徽芜湖241000 [2]北德克萨斯大学化学系,Denton 76201

出　　处：《分析测试学报》2022年第4期570-577,共8页Journal of Instrumental Analysis

基　　金：The Key Project of National Natural Science Foundation of China(21936001);The National Natural Science Foundation of China(21976004);The Anhui Province Outstanding Youth Fund(2008085J10)。

摘　　要：DNA自组装由于其低毒性、高生物相容性和内置特性,在生物传感、药物递送和临床治疗中发挥着越来越重要的作用。与大多数天然聚合物或合成纤维相比,这些DNA分子相对坚固,可通过序列变异进行修饰。作为侧链连接到聚合物上形成的二级结构赋予DNA结构可设计的响应特性,如对金属离子、蛋白质、pH、DNA、RNA和其他一些小信号分子(如ATP)的响应,可实现DNA原位自组装并进一步用于细胞荧光成像分析。该文综述了DNA自组装的最新进展,重点介绍了引发剂,并以触发机制分类探究其荧光成像应用。DNA self-assemblies play an increasingly significant role in biosensing,drug delivery and therapy due to their minimal toxicity,high biocompatibility and built-in features.Compared to most natural polymers or synthetic fi⁃bers,these DNA molecules could be relatively strong while also modifiable through sequence variation.By simply at⁃taching to a polymer as a side chain,the secondary structure formed could endow the DNA structure with designable responsiveness to something like metal ions,proteins,pH,DNA,RNA,and some other small signal molecules(such as ATP)response to achieve some functional self-assemblies.In this review,the most recent progress of DNA self-assembly is introduced.The focus is on the initiator to distinguish the trigger mechanism and their fluorescence imaging applications.

关 键 词：DNA原位自组装 荧光成像 活细胞 触发机制 

分 类 号：O657.3[理学—分析化学]