Human leukocyte antigen B polymorphism and association between HLA-B27 and endoplasmic reticulum aminopeptidase 1 rs30187 SNP in patients with ankylosing spondylitis in Bangladesh  

作　　者：Md Kamrul H.Sajib Shamim Ahmed Syed A.Haq Minhaj R.Choudhury Mohammed Uddin Furkan Uddin Muhammad S.A.Khan Mohammad M.Zaman 

机构地区：[1]Department of Rheumatology,Dhaka Medical College Hospital,Dhaka,Bangladesh [2]Department of Rheumatology,Bangabandhu Sheikh Mujib Medical Univesity,Dhaka,Bangladesh [3]Department of Human Genetics,Mohammed Bin Rashid University of Medicine and Health Sciences,College of Medicine,Dubai,UAE [4]Genetics and Genomic Department,NeuroGen Healthcare,Dhaka,Bangladesh [5]Department of Medicine,Sheikh Hasina Institute of Burn and Plastic Surgery,Dhaka,Bangladesh [6]Advisor(Research and Publication),World Health Organization,Dhaka,Bangladesh

出　　处：《Rheumatology & Autoimmunity》2022年第1期15-21,共7页风湿病与自身免疫（英文）

基　　金：Bangabandhu Sheikh Mujib Medical University。

摘　　要：Objectives:To determine the association of Human leukocyte antigen B(HLA‑B)alleles with ankylosing spondylitis and the allelic association of HLAB27 with endoplasmic reticulum aminopeptidase 1(ERAP1)single-nucleotide polymorphism(SNP)rs30187 in patients with ankylosing spondylitis(AS).Methods:We studied 48 consecutively enrolled well-defined AS patients and 48 healthy controls recruited from the outpatient clinic of the rheumatology department of Bangabandhu Sheikh Mujib Medical University(BSMMU),Dhaka,Bangladesh.To genotype HLA-B alleles from peripheral blood DNA,we designed sequence-specific primers for polymerase chain reaction.Sanger's sequencing method was used to detect the ERAP1 rs30187 SNP.The HLA-B allele distributions were compared between cases and controls.To infer an allelic association between HLA-B27 and ERAP1,allele distributions from both loci were compared using cross-tabulations.Chi-squared test or Fisher's exact test was used to quantify the association,and their strength of association was tested by odds ratio(OR)with 95%confidence interval(CI).Results:The frequency of HLA-B27 was found to be higher in AS patients(60.4%)than in healthy controls(8.3%).HLA-B27 allele had an OR of 16.8(95%CI=5.2–54.4)for the development of AS.Overall,4%of AS patients had both HLA-B27 and HLA-B40.No significant association was found between ERAP1 rs30187 SNP and HLA-B27 in patients with AS(OR=1.7,95%CI=0.5–5.6).Conclusions:HLA-B27 was significantly associated with AS in patients in Bangladesh.However,no association between ERAP1 rs30187 SNP and HLA-B27 was observed.

关 键 词：AS BANGLADESH ERAP1 rs30187 SNP HLA-B HLA-B27 

分 类 号：R59[医药卫生—内科学]