开关式氧化锌量子点荧光探针制备及其特异性检测Cu_(2+)应用  

Preparation of Switchable ZnO-NH_(2) QDs Fluorescent Probe and Its Application in Specific Detection of Cu_(2+)

作　　者：崔葆 黄秋梅 戎西林 崔美佳 程昊 冯军 黄文艺 CUI Bao;HUANG Qiu-mei;RONG Xi-lin;CUI Mei-jia;CHENG Hao;FENG Jun;HUANG Wen-yi(Key Laboratory of Green Processing of Sugar Resources in Guangxi/College of Biological and Chemical Engineering, Guangxi University of Science and Technology, Liuzhou 545006, China;Sucrose Industry Co-construction Collaborative Innovation Center, Nanning 530004, China)

机构地区：[1]广西科技大学生物与化学工程学院,广西糖资源绿色加工重点实验室,广西柳州545006 [2]蔗糖产业省部共建协同创新中心,广西南宁530004

出　　处：《发光学报》2022年第4期620-632,共13页Chinese Journal of Luminescence

基　　金：广西糖资源绿色加工重点实验室开放课题(GXTZY202006)资助项目。

摘　　要：采用溶胶凝胶法制备出一种荧光性能稳定的氧化锌量子点(ZnO-NH_(2)QDs),并对其进行紫外-可见光谱(UV-Vis)、傅里叶变换红外光谱(FTIR)、X射线衍射(XRD)、透射电子显微镜(TEM)、X射线光电子能谱(XPS)、荧光(PL)表征。基于Cu_(2+)对ZnO-NH_(2)QDs动态荧光猝灭且Cu_(2+)可与邻苯二胺(OPD,为非荧光物质)反应生成具有黄色荧光的2,3-二氨基吩嗪(DAP)的机理,开发了一种具有特异性识别Cu_(2+)的ZnO-NH_(2)QDs荧光探针以及ZnO-NH_(2)QDs+Cu_(2+)+OPD荧光传感系统。该探针在40~9000 nmol/L的线性范围内检测Cu_(2+),检测限(LOD)为3.93 nmol/L。在自来水中的加标回收率范围为98%~100.58%,在柳江水中Cu_(2+)的加标回收率范围为97.43%~101.47%。实验结果表明该探针对Cu_(2+)有较好的选择性和准确度,能为金属离子Cu_(2+)的荧光快速检测提供一种新思路。In this paper,a kind of fluorescence stable ZnO-NH_(2) QDs was prepared by sol-gel method.ZnO-NH_(2) QDs were characterized by UV-visible spectroscopy(UV-Vis),Fourier transform infrared spectroscopy(FTIR),X-ray diffraction(XRD),transmission electron microscopy(TEM),X-ray photoelectron spectroscopy(XPS),and fluorescence(PL).Based on the dynamic fluorescence quenched of ZnO-NH_(2) QDs by Cu_(2+) and the mechanism that Cu_(2+) can react with o-phenylenediamine(OPD,a non-fluorescent substance)to form 2,3-diaminophenothiazine(DAP)with yellow fluorescence,a fluorescent probe for ZnO-NH_(2) QDs with specific recognition of Cu_(2+) and a ZnO-NH_(2) QDs+Cu_(2+)+OPD fluorescence sensing system were developed.The probe detected Cu_(2+) over a linear range of 40-9000 nmol/L with detection limit of 3.93 nmol/L.The recovery rate of standard addition ranged from 98%to 100.58%in tap water,and that was 97.43%to 101.47%in Liujiang water.The experimental results show that the probe has good selectivity and accuracy for Cu_(2+),which can provide a new idea for the fluorescence rapid detection of Cu_(2+)metal ions.

关 键 词：氧化锌量子点 开关式荧光探针 Cu_(2+) 

分 类 号：O482.31[理学—固体物理] TQ132.41[理学—物理]