棉大卷叶螟求偶行为节律和性信息素鉴定  

Circadian rhythm of calling behavior and sex pheromone identification of Sylepta derogata(Lepidoptera:Pyralidae)

作　　者：朱俣伟 司玉晓 邓颖 李瑜 邓建宇[2] 董双林[1] 闫祺 ZHU Yu-Wei;SI Yu-Xiao;DENG Ying;LI Yu;DENG Jian-Yu;DONG Shuang-Lin;YAN Qi(College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China;College of Advanced Agricultural Science, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China)

机构地区：[1]南京农业大学植物保护学院,南京210095 [2]浙江农林大学现代农学院,杭州311300

出　　处：《昆虫学报》2022年第3期280-288,共9页Acta Entomologica Sinica

基　　金：国家重点研发计划“作物免疫调控与物理防控技术及产品研发”(2017YFD0200901)。

摘　　要：【目的】观察棉大卷叶螟Sylepta derogata雌蛾的求偶行为及其动态节律,并鉴定该虫的性信息素组分,为开发该虫的性诱剂并应用于种群测报和绿色防控提供依据。【方法】将棉大卷叶螟处女雌蛾单头放入塑料杯中,从暗期开始前0.5 h到暗期结束后0.5 h,每隔0.5 h观察并记录1次雌蛾的求偶情况;在棉大卷叶螟雌蛾求偶高峰期采用正己烷作为溶剂对雌蛾性信息素腺体进行浸提;利用气相色谱-触角电位联用仪(GC-EAD)确定性信息素腺体浸提液中活性组分所对应的色谱峰;利用气相色谱-质谱联用仪(GC-MS)获得性腺浸提液中活性组分的化学结构信息。【结果】棉大卷叶螟雌蛾求偶高峰期在暗期4-7.5 h之间,3-4日龄雌蛾求偶行为最为活跃;求偶时触角左右摆动,双翅快速振动,产卵器持续外伸。GC-EAD分析结果表明,雌蛾性信息素腺体浸提液中有两种组分可引起雄蛾较强的EAG反应;GC-MS分析结果表明,其中含量较高的组分为双键位于第10和12位的十六碳烯不饱和醛(10,12-16∶Ald),通过比对性信息素浸提液与含10,12-16∶Ald 4种同分异构体的标准品混合溶液的GC分析图谱进一步确定为E10,Z12-16∶Ald。进一步的触角电位测定结果显示雄蛾触角对E10,Z12-16∶Ald有显著的电位反应,且呈现一定的剂量效应。【结论】棉大卷叶螟3-4日龄雌蛾求偶最为活跃,求偶高峰期为暗期4-7.5 h之间,推测性信息素主组分为E10,Z12-16∶Ald,有待田间诱捕试验进行验证。【Aim】To observe the calling behavior and dynamic rhythm,and to identify the sex pheromone component of female moths of Sylepta derogata,so as to provide basis for the development of sex attractants and population forecast and green control of this pest insect.【Methods】The virgin female moths of S.derogata were placed individually in a plastic cup and their calling behavior was observed every half hour from 0.5 h before scotophase to 0.5 h after scotophase.The female sex pheromone glands were collected from virgin female moths during the peak calling period and extracted by hexane.The chromatographic peaks of the active components of the sex pheromone gland extracts were analyzed by gas chromatography coupled with electroantennographic detection(GC-EAD),and the chemical structure information of the active components of the sex gland extracts was determined by GC coupled with mass spectrometry(GC-MS).【Results】The peak calling period of female moths of S.derogata was between the 4th and 7.5th hour into scotophase,and the 3-4 day-old female moths displayed the most active calling behavior.When female moths were calling,their antennae swinged from side to side,their wings vibrated rapidly,and the ovipositor kept extended.Two pheromone candidates,which could elicit strong EAG response of male moths,were detected in the extracts of the female sex pheromone glands by GC-EAD analysis,and the component with higher content was 10,12-16∶Ald detected by GC-MS and further identified as(10E,12Z)-10,12-hexadecadienal(E10,Z12-16∶Ald)by comparing the results of GC analysis between the pheromone gland extracts and the blend of synthetic standard compounds containing four 10,12-16∶Ald isoforms.Further EAG test showed that E10,Z12-16∶Ald could elicit a significant EAG response of male moths in a dose-dependent manner.【Conclusions】The 3-4-day-old female moths of S.derogata show the most active calling behavior,and the peak calling period of female moths is between the 4th and 7.5th hour into scotophase,and the

关 键 词：棉大卷叶螟 求偶节律 性信息素 反10 顺12-十六碳烯醛 性诱剂 

分 类 号：Q968[生物学—昆虫学]