强化反硝化除磷的新型多级缺氧-好氧工艺  

Enhanced denitrifying phosphorus removal in novel multistage anoxic-oxic process

作　　者：卢瑞朋 徐文江 李安峰 董娜 LU Rei-peng;XU Wen-jiang;LI An-feng;Dong Na(State Environmental Protection Engineering(Beijing)Center for Industrial Wastewater Pollution Control,Beijing Municipal Research Institute of Eco-Environmental Protection,Beijing 100037,China)

机构地区：[1]北京市生态环境保护科学研究院,国家环境保护工业废水污染控制工程技术(北京)中心,北京100037

出　　处：《中国环境科学》2022年第4期1706-1713,共8页China Environmental Science

基　　金：北京市生态环境保护科学研究院基金资助项目(Y2020-004)。

摘　　要：基于多级缺氧-好氧(MAO)工艺和反硝化除磷理论,设计一种具有反硝化除磷功能的新型MAO工艺(DPR-MAO).实验探究了该工艺的脱氮除磷效能以及各反应池的微生物群落特征.工艺运行结果表明,稳定期COD、TN、NH_(4)^(+)-N和TP的平均出水浓度分别为7.07,9.04,0.34,和0.49mg/L,平均去除率分别为98%、87%、99%和93%.出水水质满足《城镇污水处理厂污染物排放标准》(GB 18918-2002)一级A标准.高通量测序结果表明,各反应池中微生物以变形菌门(Proteobacteria)、拟杆菌门(Bacteroidota)和绿弯菌门(Chloroflexi)为主,分别占61.85%~75.58%、16.39%~22.60%、1.52%~4.76%.通过属水平的研究进一步分析发现,Thiothrix、Comamonas、Candidatus Accumulibacter、和Pseudomonas是具有反硝化除磷功能的优势菌属.DPR-MAO工艺实现了反硝化聚磷菌的富集以及污水中氮磷的高效去除.Based on the multistage anoxic-oxic(MAO)process and the theory of denitrifying phosphorus removal,a noval MAO process(DPR-MAO)with ability of denitrifying phosphorus removal was designed.In this study,the nitrogen and phosphorus removal performance and the microbial community characteristics of each reaction tank were investigated.During period of stable operation,the average COD,TN,NH_(4)^(+)-N and TP concentration in effluent were 7.07,9.04,0.34 and 0.49mg/L,respectively.Meanwhile,the average removal efficiecny of COD,TN,NH_(4)^(+)-N and TP were 98%,87%,99%and 93%,respectively.The effluent quality of DPR-MAO process could meet the first A level of the"Discharge standard of pollutants for municipal wastewater treatment plant"(GB 18918~2002).High-throughput sequencing results suggested that Proteobacteria,Bacteroidetes and Chloroflexi were the dominant phyla in each reaction tank,accounting for 61.85%~75.58%、16.39%~22.60%and 1.52%~4.76%of the total phyla,respectively.Further analysis at the genus level found that Thiothrix,Comamonas,Candidatus Accumulibacter and Pseudomonas were the dominant genus for denitrifying phosphorus removal.The DPR-MAO process realized the enrichment of denitrifying phosphorus accumulating organisms and the efficient removal of nitrogen and phosphorus from wastewater.

关 键 词：反硝化除磷 多级缺氧-好氧 脱氮除磷 高通量测序 微生物群落 

分 类 号：X703.1[环境科学与工程—环境工程]