High-performance cascade nanoreactor based on halloysite nanotubes-integrated enzyme-nanozyme microsystem  

作　　者：Yan Liu Rui Lv Shiyong Sun Daoyong Tan Faqin Dong Yevgeny A.Golubev Xiaoqin Nie Olga B.Kotova Jin Liu Ke Wang 

机构地区：[1]School of Environment and Resource,Key Laboratory of Solid Waste Treatment and Resource Recycle of Ministry of Education,Southwest University of Science and Technology,Mianyang 621010,China [2]Yushkin’s Institute of Geology,Komi Science Center,Ural Branch of RAS,ul.Pervomayskaya,54,Syktyvkar 167982,Russia

出　　处：《Chinese Chemical Letters》2022年第2期807-811,共5页中国化学快报（英文版）

基　　金：supported by the National Natural Science Foundation of China (NSFC,Nos.42061134018,42011530085 and 41877323);the Russian Science Foundation (RSF,No.21-4700019,Russia);the Sichuan Science and Technology Program (No.2019JDJQ0056,China)。

摘　　要：Various enzymatic reactions or enzymatic cascade reactions occur efficiently in biological microsystems due to space constraints or orderly transfer of intermediate products. Inspired by this, the horseradish peroxidase(HRP)-like nanozyme(Fe-aminoclay) was in situ synthesized on the surface of alkali-activated halloysite nanotubes and the natural enzyme(glucose oxidase, GOx) was immobilized on it to construct a high-efficiency GOx-Fe AC@AHNTs cascade nanoreactor. In which, Fe AC@AHNTs can not only be used as a carrier for immobilized enzymes, but also help its catalytic activity to cooperate with glucose oxidase in a cascade reaction. The microcompartments and substrate channel effect of this enzyme-nanozyme microsystem exhibit a superior catalytic performance than that of natural enzyme system, and exhibits excellent long-term stability and recyclability. Subsequently, the GOx-Fe AC@AHNTs cascade nanoreactor was employed as a glucose colorimetric platform, which displayed a low detection limit(0.47 μmol/L)in glucose detection. This enzyme-nanoenzyme nanoreactor provides a simple and effective example for constructing a multi-enzyme system with limited space, and lays the foundation for subsequent research in the fields of biological analysis and catalysis.

关 键 词：Halloysite nanotubes Fe-aminoclay Nanozyme Cascade nanoreactor Catalysis enhancement 

分 类 号：TQ426.97[化学工程]