齐墩果酸对癫痫发作的作用研究  

Study on the Effect of Oleanolic Acid on Epileptic Seizure

作　　者：刘昆梅[1,2] 黄越 万盼盼 周宁 张莲香 强媛媛[1] 郭乐 LIU Kun-mei;HUANG Yue;WAN Pan-pan;ZHOU Ning;ZHANG Lian-xiang;QIANG Yuan-yuan;GUO Le(Ningxia Key Laboratory of Cerebrocranial Diseases,Yinchuan 750004,China;Medical Science Research Institution of Ningxia Hui Autonomous Region,Yinchuan 75004,China;Clinical Medical College of Ningxia Medical University,Yinchuan 750004,China;School of Pharmacy,Ningxia Meadical University,Yinchuan 750004,China)

机构地区：[1]宁夏医科大学宁夏颅脑疾病重点实验室,宁夏银川750004 [2]宁夏回族自治区医学科学研究所,宁夏银川750004 [3]宁夏医科大学宁夏临床医学院,宁夏银川750004 [4]宁夏医科大学药学院,宁夏银川750004

出　　处：《海峡药学》2022年第3期35-39,共5页Strait Pharmaceutical Journal

基　　金：宁夏自然科学基金(2018AAC0218);国家自然科学基金(32070930)。

摘　　要：目的研究齐墩果酸抗癫痫作用,及其在癫痫过程中的抗炎抗氧化神经保护作用。方法取雄性大鼠随机分为对照组、癫痫模型组和癫痫并齐墩果酸给药低、中、高剂量组(分别为20 mg/(kg·d)、50 mg/(kg·d)、100 mg/(kg·d),ig给药)。采用癫痫行为观察方法比较大鼠癫痫发作潜在时间来评价齐墩果酸的抗癫痫作用,并通过ELISA实验研究其抗炎抗氧化作用。结果与模型组相比,齐墩果酸中剂量和高剂量给药组癫痫发作的潜伏时间显著增加,齐墩果酸高剂量给药组炎症因子IL-1β和TNF-α,以及氧化应激标志物8-OHdG表达量均显著降低。结论齐墩果酸具有抗癫痫作用,其可能机制与抗炎抗氧化神经保护作用有关。OBJECTIVE To study the anti-epileptic effect of oleanolic acid and its anti-inflammatory and anti-oxidative effects during the process of epilepsy.METHODS Male rats were randomly divided into control group,epilepsy model group and oleanolic acid low-dose,medium-dose and high-dose group(20 mg/(kg·d),50 mg/(kg·d),100 mg/(kg·d),respectively).The antiepileptic effect of oleanolic acid was evaluated by comparing the latency of seizures of rats after LiCl-PILO injection.The anti-inflammatory and anti-oxidative effect of oleanolic acid was studied by ELISA method.RESULTS Compared with the epilepsy model group,the latency of seizures of oleanolic acid medium-dose and high-dose group increased significantly,and the concentration of IL-1β,TNF-α and 8-OHDG in the high dose administration group were significantly higher than epilepsy model group.CONCLUSION Oleanolic acid had anti-epileptic effect,and its possible mechanism is related to anti-inflammatory anti-oxidative effect.

关 键 词：齐墩果酸 癫痫 炎症 氧化应激 

分 类 号：R965[医药卫生—药理学]