鱼腥草素钠联合亚胺培南西司他丁钠对碳青霉烯耐药鲍曼不动杆菌体外抑菌作用的研究  

Antibacterial effects of sodium houttuyfonate combined with imipenem cilastatin sodium against carbapenem-resistant Acinetobacter baumannii in vitro

作　　者：周孟杰 毛建梅 蔡燕[2,3] ZHOU Mengjie;MAO Jianmei;CAI Yan(Department of Laboratory Medicine,Affiliated Hospital of North Sichuan Medical College,Nanchong,Sichuan 637000,China;Prenatal Diagnosis Center,Affiliated Hospital of North Sichuan Medical College,Nanchong,Sichuan 637000,China;Institute of Rheumatism and Immunology,Affiliated Hospital of North Sichuan Medical College,Nanchong,Sichuan 637000,China)

机构地区：[1]川北医学院附属医院检验科,四川南充637000 [2]川北医学院附属医院产前诊断中心,四川南充637000 [3]川北医学院附属医院风湿免疫研究所,四川南充637000

出　　处：《重庆医学》2022年第7期1122-1126,1130,共6页Chongqing medicine

基　　金：四川省卫生健康委员会科研课题(20PJ145);2019年南充市市校科技战略合作专项(19SXHZ0291)。

摘　　要：目的探讨鱼腥草素钠联合亚胺培南西司他丁钠对碳青霉烯耐药鲍曼不动杆菌(CRAB)的联合抑菌作用。方法选取10株CRAB为实验对象,采用微量肉汤稀释法、琼脂二倍稀释法测定鱼腥草素钠、亚胺培南西司他丁钠单药及联合用药对CRAB菌株生长的影响。结果鱼腥草素钠能抑制CRAB生长,微量肉汤稀释法测得鱼腥草素钠对CRAB的最低抑菌浓度(MIC)在1500~3500μg/mL;琼脂二倍稀释法测得鱼腥草素钠对CRAB的最低抑菌浓度在2500~3500μg/mL;2种方法检测鱼腥草素钠与亚胺培南西司他丁钠的部分抑菌浓度指数(FICI)均在0.5~1.0,抑菌效应表现为相加作用。结论鱼腥草素钠能抑制CRAB菌株生长,与亚胺培南西司他丁钠联合具有相加作用。Objective To explore the combined antibacterial effect of sodium houttuyfonate combined with imipenem cilastatin sodium on carbapenem-resistant acinetobacter baumannii(CRAB).Methods A total of ten clinical strains of CARB were selected as study subjects.Microdilution broth method and double agar dilution method were used to detect the effects of sodium houttuyfonate and imipenem cilastatin sodium and their combination on the growth of the CRAB strain.Results Sodium houttuyfonate had a bacteriostatic effect on the CRAB.The minimum inhibitory concentration(MIC)of sodium houttuyfonate of the microdilution broth method was 1500-3500μg/mL,while that of the double agar dilution method was 2500-3000μg/mL.The fractional inhibitory concentration index(FICI)of sodium houttuyfonate and imipenem cilastatin sodium detected by the two methods were 0.5-1.0,and the bacteriostatic effect showed a addictive effect.Conclusion Sodium houttuyfonate has the bacteriostasis against effect on CRAB and has an addictive effect with imipenem cilastatin sodium.

关 键 词：鱼腥草素钠 亚胺培南西司他丁钠 耐碳青霉烯鲍曼不动杆菌 相加作用 

分 类 号：R446.5[医药卫生—诊断学]