玉米泛素连接酶U-box基因家族的全基因组鉴定及表达分析  

Genome-wide identification and expression analysis of ubiquitin ligase U-box gene family in maize

作　　者：陈曙[1,2] 张彧 陈卓 金辉 CHEN Shu;ZHANG Yu;CHEN Zhuo;JIN Hui(South Subtropical Crops Research Institute,Chinese Academy of Tropical Agricultural Sciences,Zhanjiang,Guangdong 524091,China;Key Laboratory of Tropic Fruit Biology,Ministry of Agriculture,Zhanjiang,Guangdong 524091,China)

机构地区：[1]中国热带农业科学院南亚热带作物研究所,广东湛江524091 [2]农业部热带果树生物学重点实验室,广东湛江524091

出　　处：《西南农业学报》2022年第3期481-490,共10页Southwest China Journal of Agricultural Sciences

基　　金：中央级非盈利性科研院所基本科研业务费(1630062017015)。

摘　　要：【目的】U-box基因家族广泛存在于植物基因组中,能够编码泛素蛋白酶体系中特异性识别底物的泛素E3连接酶,调控蛋白的修饰和降解,对玉米的生长发育及逆境胁迫应答调控中起着重要作用,因此鉴定玉米U-box基因家族成员及进行基因功能研究具有重要意义。【方法】通过玉米全基因组数据库,利用一系列生物信息学工具对玉米U-box基因家族成员进行鉴定和功能分析。【结果】玉米U-box基因家族共鉴定筛选出76个成员,在玉米全基因组1~10号染色体上均有分布,氨基酸数目大小为94~1353 aa,蛋白等电点数值差距较大,从4.86到8.29不等。基因结构分析结果显示各成员间含有内含子数目不等,其中玉米U-box家族76个成员中共有28个基因无内含子,仅含有1个外显子,占玉米U-box基因总数的36.8%,表明这些基因进化可能源于转座子机制。系统进化分析结果显示水稻、拟南芥、玉米U-box基因分布在亚家族Ⅰ~Ⅸ中,其中亚家族Ⅳ中不含水稻、拟南芥U-box成员,仅含有2个玉米基因ZmPUB41和ZmPUB53,暗示了玉米在历史进化过程中在发生了多次家族基因扩增的同时也经过片段快速变异过程。启动子功能预测分析发现玉米U-box基因在玉米光合作用、植物激素应答以及逆境胁迫等方面发挥相应作用。基因组织表达分析显示玉米U-box基因表达特异性明显,主要在种子的组成型器官中表达,暗示了目标基因可能参与了种子的形成或萌发过程。部分基因如ZmPUB12、ZmPUB18、ZmPUB30、ZmPUB40、ZmPUB75仅在花粉中检测出有表达量,说明其可能参与了玉米花粉粒的形成或受精过程。【结论】玉米U-box基因在玉米的光合作用、生长发育以及逆境胁迫应答等生理活动中发挥了重要作用,为后续对玉米U-box基因功能、蛋白代谢和信号转导等重要调控网络研究提供了理论基础。【Objective】The U-box gene family widely exists in plant genomes and can encode ubiquitin E3 ligase that specifically recognizes substrates in the ubiquitin protease system,regulates protein modification and degradation,and responds to corn growth and stress.So it is of great significance to identify the members of the maize U-box gene family and conduct gene function studies.【Method】By the maize whole genome database,the present study used a series of bioinformatics tools to identify and functionally analyze the members of the maize U-box gene family.【Result】A total of 76 members of the maize U-box gene family were identified and screened,and they were distributed on chromosomes 1-10 of the maize genome.The number of amino acids was 94-1353 aa,and the isoelectric point value of the protein was quite different.It varied from 4.86 to 8.29.The results of gene structure analysis showed that the number of introns varied among the members.Among the 76 members of the maize U-box family,28 genes had no introns and only contained 1 exon,accounting for the total number of maize U-box genes.36.8%of these genes indicated that the evolution of these genes may be derived from the transposon mechanism.The results of phylogenetic analysis showed that U-box genes of rice,arabidopsis and maize were distributed in subfamily I-IX.Subfamily IV did not contain rice and arabidopsis U-box members,but contained only 2 maize genes.ZmPUB41 and ZmPUB53 implied that maize had undergone multiple family gene amplifications in the course of historical evolution and also undergone rapid fragment mutation.Promoter function prediction analysis found that the maize U-box gene played a corresponding role in maize photosynthesis,plant hormone response,and adversity stress.The analysis of gene tissue expression showed that the maize U-box gene was expressed specifically in the constitutive organs of seeds,suggesting that these genes may be involved in the formation or germination of seeds.Some genes such as ZmPUB12,ZmPUB18,ZmPUB30,ZmPUB40,

关 键 词：玉米 泛素U-box基因家族 生物信息学 启动子功能分析 基因表达 

分 类 号：S513[农业科学—作物学]