铜锌交互效应对方格星虫稚虫生长和生理机能的影响  

Effects of Cu-Zn interaction on growth and physiological function of juvenile peanut worm, Sipunculus nudus Linnaeus

作　　者：许明珠[1] 董兰芳[1] 李恒慧 李世才 陈瑞芳 张琴 彭慧婧 龙子扬 林立蔚 XU Ming-zhu;DONG Lan-fang;LI Heng-hui;LI Shi-cai;CHEN Rui-fang;ZHANG Qin;PENG Hui-jing;LONG Zi-yang;LIN Li-wei(Guangxi Institute of Oceanology Co./Guangxi Engineering Technology Research Center of Breeding of New Mariculture Varieties,Beihai,Guangxi 536000,China;Hepu Aquatic Technology Extension Station,Hepu,Guangxi 536100,China;Guangxi Academy of Sciences,Nanning 530007,China;Guangxi University for Nationalities,School of Biotechnology and Marine Science/Guangxi Laboratory of Polysaccharide Materials and Modification/Guangxi Colleges and Universities Key Laboratory of Utilization of Microbial and Botanical Resources,Nanning 530006,China)

机构地区：[1]广西海洋研究所有限责任公司/广西海水养殖新品种繁育工程技术研究中心,广西北海536000 [2]合浦县水产技术推广站,广西合浦536100 [3]广西科学院,南宁530007 [4]广西民族大学海洋与生物技术学院/广西多糖材料与改性实验室/广西高校微生物与植物资源利用重点实验室,南宁530006

出　　处：《西南农业学报》2022年第3期721-728,共8页Southwest China Journal of Agricultural Sciences

基　　金：广西创新驱动发展专项(桂科AA17204044);广西科学院基本科研业务费项目(2018YJJ904)。

摘　　要：【目的】明确微量元素铜锌交互效应对方格星虫稚虫生长和生理机能的影响,为开发方格星虫高效环保人工配合饲料提供参考依据。【方法】设计3×3双因素试验,将平均体重为(14.32±0.21)mg的方格星虫稚虫8100条随机分为9组,基础饲料分成3组,每组分别添加实际铜含量5.0、10.0和20.0 mg/kg及实际锌含量20.0、40.0和80.0 mg/kg,制成9种饲料(处理1~处理9),进行8周的方格星虫稚虫养殖试验,分析评价不同处理方格星虫稚虫的生长和生理机能。【结果】饲料中微量元素铜锌对方格星虫稚虫增重率、特定生长率、成活率、碱性磷酸酶(AKP)活性、虫体铜和虫体锌含量均具有显著影响(P<0.05,下同),对其体成分无显著影响(P>0.05,下同)。饲料中的铜和锌添加量对方格星虫稚虫增重率、特定生长率、成活率和AKP活性具有显著交互效应,对其体成分、虫体铜和虫体锌含量无交互效应。处理8(铜添加量为20.0 mg/kg,锌添加量为40.0 mg/kg)方格星虫稚虫的增重率和特定生长率最高;锌添加量为40.0 mg/kg处理组(处理2、处理5和处理8)方格星虫稚虫的增重率和特定生长率均显著高于锌添加量为80.0 mg/kg的处理组(处理3、处理6和处理9);当铜添加量适量或偏低(5.0和10.0 mg/kg)时,方格星虫稚虫的成活率主要受锌含量影响,表现为随锌含量的升高呈先升高后降低的变化趋势,而当铜添加量上升至20.0 mg/kg时,方格星虫稚虫的存活率主要受铜添加量影响,但处理间差异不显著;方格星虫稚虫的AKP活性主要受饲料中锌含量影响,其中,在添加锌水平为20.0 mg/kg的处理组(处理1、处理4和处理7)中较低,且显著低于其余处理;虫体铜、锌含量同样受饲料铜、锌添加量的影响,与饲料中铜、锌添加量的变化呈正相关。【结论】饲料中铜和锌添加量均可影响方格星虫稚虫生长、体成分和体内元素的分布和积累,以及AKP活性,其中饲料�【Objective】This trial was conducted to investigate the effects of Cu-Zn Interaction on growth and physiological function of juvenile peanut worm, Sipunculus nudus Linnaeus.It provided a reference for the development of efficient and environmentally friendly artificial compound feed for S.nudus.【Method】It was a 3×3 two-factor experiment.8100 S.nudus juveniles with average body weight of(14.32±0.21)mg were randomly divided into 9 groups, and the basic feed was divided into three groups.In each group, copper methionine with actual copper content of 5.0,10.0 and 20.0 mg/kg and zinc methionine with actual zinc content of 20.0,40.0 and 80.0 mg/kg were added respectively.9 kinds of feeds(treatment 1-treatment 9) were fed to S.nudus for 8 weeks.The growth and physiology of different treatments were evaluated.【Result】The interaction of trace elements Cu and Zn in the feed had significant effects on the weight gain rate, specific growth rate, survival rate, AKP,copper and zinc content of the larvae(P<0.05,the same as below),but had no significant effect on body composition(P>0.05,the same as below).Moreover, the additive amount of copper and zinc in feed had significant interactive effects on weight gain rate, specific growth rate, survival rate and alkaline phosphatase activity, but had no interactive effects on the body composition, copper content and zinc content of S.nudus.Test group 8(Cu: 20.0 mg/kg, Zn: 40.0 mg/kg) had the highest weight gain rate and specific growth rate.The weight gain rate and specific growth rate of the treatment groups with zinc content of 40.0 mg/kg(treatment 2,treatment 5 and treatment 8) were significantly higher than those of the treatment groups with zinc content of 80.0 mg/kg(treatment 3,treatment 6 and treatment 9).When the copper content was low(5.0 mg/kg, 10.0 mg/kg),the survival rate of S.nudus juveniles is mainly affected by zinc, and with the increase of zinc content, the experimental groups showed a trend of first increasing and then decreasing.When the content of copp

关 键 词：方格星虫 铜锌交互效应 饲料 生长 生理机能 

分 类 号：S963.1[农业科学—水产养殖]