鳕鱼皮源医用复合止血敷料的制备及性能研究  

Preparation and properties of medical compound hemostasis dressing from Pacific cod skin

作　　者：宋雪 刘晗[1] 李昭璇 李八方[1] 侯虎[1,2] SONG Xue;LIU Han;LI Zhaoxuan;LI Bafang;HOU Hu(College of Food Science and Engineering,Ocean University of China,Qingdao 266003,China;Qingdao Pilot National Laboratory for Marine Science and Technology,Qingdao 266237,China)

机构地区：[1]中国海洋大学食品科学与工程学院,山东青岛266003 [2]青岛海洋科学与技术试点国家实验室,山东青岛266237

出　　处：《南方水产科学》2022年第2期66-73,共8页South China Fisheries Science

基　　金：国家重点研发计划项目(2019YFD0901905);山东省泰山学者项目(tsqn202103033)。

摘　　要：以太平洋鳕(Gadus macrocephalus)鱼皮明胶为原料,通过复配海藻酸钠得到一种可吸收复合止血敷料,并对其机械性能、止血效果、止血机理和生物相容性进行评价。结果表明,明胶质量浓度为10 mg·mL^(-1)时制备的止血敷料具有较高的机械强度。体内止血评价结果显示,复合止血敷料对大鼠股动脉止血、肝创面止血和断尾止血模型均有良好的效果,止血时间分别为(64±9)、(108±4)和(230±25)s,与市售明胶海绵的止血效果相当。复合止血敷料可缩短活化部分凝血活酶时间(Activated partial thromboplastin time,APTT)和凝血酶时间(Thrombin time,TT),激活内源性凝血系统和共同性凝血途径,以及促进血栓烷素B;(TXB;)、血小板第四因子(PF4)和P-选择素的释放,达到快速止血效果。生物相容性实验结果证实,该复合敷料无急性毒性、无刺激性且溶血率小于5%,达到医用材料的行业标准。该研究结果为开发新型医用敷料并实现鳕鱼皮高值化利用提供了参考和理论依据。In this study,an absorbable composite hemostatic dressing was prepared by combining sodium alginate with Pacific cod(Gadus macrocephalus)skin gelatin,and its mechanical properties,hemostatic effect,hemostatic mechanism and biocompatibility were evaluated.The results show that the composite hemostatic dressing had high mechanical strength with 1%of gelatin concentration.Results of hemostatic evaluation in vivo indicate that the dressing had good hemostatic effect on the femoral artery,liver wound and tail hemostasis models of rats,and the hemostatic time was(64±9),(108±4)and(230±25)s,respectively,which was similar with the hemostatic effect of commercial gelatin sponge.The dressing could shorten activated partial thromboplastin time(APTT)and thrombin time(TT),which activated endogenous coagulation and common coagulation pathway,and promoted the release of thromboxane-B;(TXB;),platelet factor-4(PF4)and P-selectin to achieve rapid hemostatic effect.Biocompatibility experiments show that the composite dressing had no acute toxicity or irritation and the hemolysis rate was less than 5%,which meets the standards of medical materials.The study provides reference and theoretical basis for developing new medical dressing and realizing high-value utilization of Pacific cod skin.

关 键 词：鳕鱼皮明胶 海藻酸钠 复合止血海绵 生物相容性 止血性能 

分 类 号：R318.08[医药卫生—生物医学工程]