不同工艺海带提取物中植物激素和海藻酸寡糖的比较研究  

Comparative research of plant hormones and alginate oligosaccharides in seaweed extracts by different processing methods

作　　者：苗钧魁[1] 张雅婷 张圣国 郑志红 王致鹏 王海英[1] MIAO Junkui;ZHANG Yating;ZHANG Shengguo;ZHENG Zhihong;WANG Zhipeng;WANG Haiying(Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences/Laboratory for Marine Drugs and Biological Products,Pilot National Laboratory for Marine Science and Technology,Qingdao 266071,China;Qingdao University of Science and Technology,Qingdao 266061,China;Hebei Agricultural Project Planning Center,Shijiazhuang 050000,China;College of Food Science and Engineering,Dalian Ocean University,Dalian 116023,China;School of Marine Science and Engineering,Qingdao Agricultural University,Qingdao 266109,China)

机构地区：[1]中国水产科学研究院黄海水产研究所/青岛海洋科学与技术试点国家实验室海洋药物与生物制品功能实验室,山东青岛266071 [2]青岛科技大学,山东青岛266061 [3]河北省农业项目规划中心,河北石家庄050000 [4]大连海洋大学食品科学与工程学院,辽宁大连116023 [5]青岛农业大学海洋科学与工程学院,山东青岛266109

出　　处：《南方水产科学》2022年第2期142-149,共8页South China Fisheries Science

基　　金：国家重点研发计划项目(2019YFD0901902);河北省重点研发计划项目(21323201D)。

摘　　要：在海带(Laminaria japonica)提取加工领域,生物酶解法已成为理想的化学法替代工艺。为评估化学、酶解和酶解发酵联合工艺的提取效果,对3种海带提取物中的植物激素和海藻酸寡糖含量以及分子量分布情况进行了比较研究。采用间羟基二苯法测定海藻酸盐质量百分比,高效液相色谱法(HPLC)测定海藻酸寡糖分子量,高效液相色谱-串联质谱法(HPLC-MS)测定植物激素含量。结果显示,化学提取法获得的海带提取物的海藻酸寡糖提取率最低(41.99%),分子量约为200~400 D,而酶解提取法和酶解发酵联合提取法获得的提取率分别为90.75%和82.21%,分子量为200~1600 D。吲哚乙酸(IAA)是3种提取物中质量分数最高的植物激素(2.64~64.59 ng·g^(-1)),其次为茉莉酸(JA,0.05~13.09 ng·g^(-1))。酶解发酵联合法海带提取物的植物激素浓度高于其他2种提取物。综合比较植物激素和海藻酸寡糖提取率,酶解发酵联用工艺比传统化学工艺和酶解工艺更适用于农用海带提取物的制备。In seaweed(Laminaria japonica)extraction processing,biological enzymolysis processing has been an ideal alternative to traditional chemical extraction method.In order to evaluate the extraction effects of three different processing methods,we treated the seaweed by chemical processing(CP),enzymolysis processing(EP)as well as combined enzymolysis and microbial fermentation processing(CEMP).Besides,we compared the contents of plant hormones and algiante oligosaccharides in addition to the molecular mass distribution in three kinds of seaweed extracts.We determined the alginate content,molecular mass of alginate oligosaccharide and plant hormones contents by m-hydroxydiphenyl method,HPLC and HPLC-MS,respectively.The results show that the CP method had the lowest extraction rate of alginate oligosaccharides(41.99%)with molecular mass of 200-400 D,while the EP method and CEMP method had relatively higher extraction rate of alginate oligosaccharides(90.75%and 82.21%)with molecular mass of 200-1600 D.Indoleacetic acid(IAA)was the most abundant plant hormone among all the three extracts,ranging from 2.64 to 64.59 ng·g^(-1),followed by jasmonic acid(JA,0.05-13.09 ng·g^(-1)).The plant hormone content of the extract by CEMP method was higher than that of the other two extracts.Based on the comprehensive comparison of extraction rate of plant hormones and alginate oligosaccharides,the CEMP method is more suitable for the preparation of agricultural seaweed extract than the CP method and EP methods.

关 键 词：海带提取物 海带 酶解 植物激素 海藻酸寡糖 

分 类 号：Q189[生物学—神经生物学]