血清miR-34b-5p、miR-155表达与早产儿急性呼吸窘迫综合征炎症因子和预后的关系分析  

Relationship Analysis between Expression of Serum miR-34b-5p and miR-155 and Inflammatory Factors and Prognosis of Premature Infants with Acute Respiratory Distress Syndrome

作　　者：陈前 张恩胜 芦庆花 张沛佩 黄磊 CHEN Qian;ZHANG En-sheng;LU Qing-hua;ZHANG Pei-pei;HUANG Lei(Department of Neonatal Pediatrics,Shandong Maternal and Child Health Hospital,Ji'nan,Shandong,250014,China;Department of Pediatrics,Shandong Maternal and Child Health Hospital,Ji'nan,Shandong,250014,China)

机构地区：[1]山东省妇幼保健院新生儿科,山东济南250014 [2]山东省妇幼保健院儿科,山东济南250014

出　　处：《现代生物医学进展》2022年第5期945-949,共5页Progress in Modern Biomedicine

基　　金：山东省医药卫生科技发展计划项目(2019WSB03007)。

摘　　要：目的:探讨血清微小核糖核酸(mi R)-34b-5p、mi R-155表达与早产儿急性呼吸窘迫综合征(ARDS)炎症因子和预后的关系。方法:选择2019年2月至2021年3月我院收治的92例ARDS早产儿,根据ARDS病情严重程度将其分为轻度组(31例)、中度组(43例)和重度组(18例),追踪患儿临床结局,根据院内死亡情况将其分为存活组(51例)和死亡组(41例)。检测所有患儿的血清mi R-34b-5p、mi R-155表达水平以及白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)水平,比较各组间上述指标差异,分析ARDS早产儿血清mi R-34b-5p、mi R-155表达与炎症因子的相关性以及mi R-34b-5p、mi R-155预测ARDS早产儿预后的价值。结果:重度组mi R-155表达水平及IL-1β、TNF-α、IL-6水平均高于中度组和轻度组,且中度组高于轻度组(P<0.05),重度组mi R-34b-5p表达水平低于中度组和轻度组,且中度组低于轻度组(P<0.05)。死亡组mi R-155表达水平及IL-1β、TNF-α、IL-6水平高于存活组(P<0.05),死亡组mi R-34b-5p表达水平低于存活组(P<0.05)。ARDS早产儿mi R-155表达水平与IL-1β、TNF-α、IL-6水平均呈正相关,而mi R-34b-5p表达水平与IL-1β、TNF-α、IL-6水平均呈负相关(P<0.05)。联合mi R-34b-5p、mi R-155预测ARDS早产儿死亡的曲线下面积(AUC)为0.853,高于两指标单独预测的0.688、0.649。结论:ARDS早产儿血清mi R-34b-5p、mi R-155表达水平与患儿血清炎症因子水平以及预后有关,可作为ARDS早产儿病情评估以及预后预测的潜在指标。Objective:To investigate the relationship between the expression of serum micro ribonucleic acid(mi R)-34b-5p and mi R-155 and the inflammatory factors and prognosis of premature infants with acute respiratory distress syndrome(ARDS).Methods:92premature infants with ARDS who were admitted to our hospital from February 2019 to March 2021 were selected,and they were divided into mild group(31 cases),moderate group(43 cases)and severe group(18 cases)according to the severity of ARDS,follow up the clinical outcome of children,according to the death in hospital,they were divided into survival group(51 cases)and death group(41 cases).The expressions of serum mi R-34b-5p and mi R-155,as well as interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)in all the children were detected.The differences of the above indexes among the groups were compared,the correlation between the expressions of serum mi R-34b-5p and mi R-155 and inflammatory factors and the value of mi R-34b-5p and mi R-155 in predicting the prognosis of premature infants with ARDS were analyzed.Results:The expression levels of serum mi R-155 and the levels of IL-1β,TNF-αand IL-6 in the severe group were higher than those in the moderate group and mild group,and those in moderate group were higher than those in mild group(P<0.05).The expression level of serum mi R-34b-5p in the severe group was lower than that in moderate and mild groups,and that in moderate group was lower than that in mild group(P<0.05).The expression level of serum mi R-155 in the death group was higher than that in the survival group(P<0.05),and the expression level of mi R-34b-5p in the death group was lower than those in the survival group(P<0.05).The expression level of mi R-155 was positively correlated with the levels of IL-1β,TNF-αand IL-6 in premature infants with ARDS(P<0.05),and the expression level of mi R-34b-5p was negatively correlated with the levels of IL-1β,TNF-αand IL-6(P<0.05).The area under curve(AUC)of mi R-34b-5p and mi R-155 predicting

关 键 词：早产儿 急性呼吸窘迫综合征 mi R-34b-5p mi R-155 炎症因子 预后 

分 类 号：R714.21[医药卫生—妇产科学]