Mechanism of calcium hydroxide inhibiting methicillin resistant Staphylococcus aureus  

作　　者：Zheng-Dong Guo Yang-Yang Bian Xiao-Qian Liu Dong Wang Si-Yuan Zhang Jian Yang Lei Peng 

机构地区：[1]Trauma medical center of the First Affiliated Hospital of Hainan Medical College,Haikou,Hainan 570216,China [2]Key Laboratory of Emergency and Trauma Ministry of Education,Hainan Medical University,Haikou,Hainan 570216,China [3]Key Laboratory of Hainan Trauma and Disaster Rescue,The First Affiliated Hospital of Hainan Medical University,Haikou,Hainan 570216,China

出　　处：《Journal of Hainan Medical University》2022年第5期18-22,共5页海南医学院学报（英文版）

基　　金：National Natural Science Foundation of China(No.82060347)。

摘　　要：Objective:To investigate the inhibitory effect of calcium hydroxide on methicillin-resistant Staphylococcus aureus and the related inhibition mechanism.Methods:To determine the minimum inhibitory concentration of calcium hydroxide using microplate dilution method;to compare the effects of calcium hydroxide at 8MIC,MIC,1/4MIC and 0 concentrations on MRSA using growth curve method;to determine the effects of calcium hydroxide on the cell membrane of methicillin-resistant Staphylococcus aureus using calcium xanthophyll and propidium iodide fluorescence staining The effect of calcium hydroxide on the morphology of methicillin-resistant Staphylococcus aureus was observed by scanning electron microscopy;the inhibition mechanism of calcium hydroxide on MRSA was investigated by sodium dodecyl sulfate polyacrylamide gel electrophoresis of protein bands.Results:The MIC of calcium hydroxide on MRSA was 3.125 mg/mL;the fluorescence intensity showed significant changes after co-culture of calcium hydroxide with bacteria;MRSA appeared to be significantly crumpled and broken in the presence of calcium hydroxide;the SDS-PAGE experimental bands indicated that the protein inside the bacteria decreased accordingly with the increase of calcium hydroxide concentration.Conclusion:Calcium hydroxide has a significant inhibitory effect on the growth of MRSA,and its bactericidal mechanism may be related to the destruction of bacterial body structure.

关 键 词：Calcium hydroxide Methicillin-resistant staphylococcus MRSA BACTERIOSTASIS Antibacterial mechanism Cytomembrane 

分 类 号：O61[理学—无机化学]