三七总皂苷对流产大鼠子宫出血的影响及子宫内膜修复作用机制的研究  

Study on the mechanism of Panax notoginseng saponins on repairing uterine bleeding and endometrium in aborted rats

作　　者：张靖 李潇 ZHANG Jing;LI Xiao(Department of Obstetrics and Gynecology,Tianjin Binhai New Area Hospital of Traditional Chinese Medicine,Tianjin 300451,China)

机构地区：[1]天津市滨海新区中医医院妇产科,300451

出　　处：《天津医药》2022年第4期375-380,共6页Tianjin Medical Journal

摘　　要：目的探究三七总皂苷对流产大鼠子宫出血的影响及可能的作用机制。方法取未怀孕雌性大鼠10只为空白对照组,受孕的雌性大鼠采用米非司酮(8.3 mg/kg)和米索前列醇(100μg/kg)诱导建立异常子宫出血(AUB)模型,随机分为模型组、阳性组(断血流片,0.45 g/kg)、三七总皂苷低(25 mg/kg)、中(50 mg/kg)、高(100 mg/kg)剂量组,每组10只。阳性组、三七总皂苷各剂量组分别给予相应剂量的药物灌胃,1次/d,共7 d。紫外分光光度法测定大鼠子宫出血量;酶联免疫吸附试验(ELISA)检测血浆肿瘤坏死因子(TNF)-α、白细胞介素(IL)-6、雌二醇(E2)、孕酮(P)、6-酮前列腺素F1α(6-keto-PGF1α)以及血栓素B2(TXB2)水平;HE、Masson染色观察子宫内膜的病理损伤;免疫荧光染色检测子宫内膜组织中微血管密度(MVD);实时荧光定量PCR(qPCR)检测子宫组织血管内皮生长因子(VEGF)、基质金属蛋白酶(MMP)-2、MMP-9 mRNA的表达。结果与空白对照组比较,模型组大鼠血浆6-ketoPGF1α、TNF-α、IL-6水平、子宫内膜组织的纤维化面积比、MMP-2和MMP-9 mRNA表达增加,TXB2、E2、P水平、子宫内膜厚度、腺体数量、子宫内膜MVD、VEGF mRNA表达降低(P<0.05);与模型组比较,三七总皂苷高剂量组大鼠子宫出血量、血浆6-keto-PGF1α、TNF-α、IL-6水平、子宫内膜组织的纤维化面积比、MMP-2和MMP-9 mRNA降低,TXB2、E2水平、子宫内膜厚度、腺体数量、子宫内膜MVD、VEGF mRNA表达增加(P<0.05)。结论高剂量三七总皂苷可治疗流产大鼠子宫出血,其作用机制可能与抑制过度纤维化和炎症,上调VEGF的表达,促进血管重塑,进而促进子宫内膜修复有关。ObjectiveTo explore the effect of Panax notoginseng saponins on uterine bleeding in aborted rats and its possible mechanism.MethodsTen non-pregnant female rats were taken as the blank control group.Female rats conceived on the same day were induced with mifepristone(8.3 mg/kg)and misoprostol(100μg/kg)to establish abnormal uterine bleeding(AUB)model,and were divided into the model group,the positive group(Duanxueliu tablets,0.45 g/kg),the Panax notoginseng saponins low-(25 mg/kg),medium-(50 mg/kg),high-(100 mg/kg)dose groups,with 10 rats in each group.The positive group and the Panax notoginseng saponins groups were given corresponding doses of drugs by gavage,once a day,for7 days.Ultraviolet spectrophotometry was used to measure the amount of uterine bleeding in rats.Enzyme-linked immunosorbent assay(ELISA)was used to detect the levels of plasma tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),estradiol(E2),progesterone(P)and 6-ketoprostaglandin F1α(6-keto-PGF1α)and thromboxane B2(TXB2).HE and Masson staining methods were used to observe the pathological damage of the endometrium.Immunofluorescence staining was used to detect microvessel density(MVD)in endometrial tissue.Real-time fluorescent quantitative PCR(qPCR)was used to detect the expression of vascular endothelial growth factor(VEGF),matrix metalloproteinase-2(MMP-2)and matrix metalloproteinase-9(MMP-9)mRNA in uterine tissue.Results keto-PGF1α,TNF-α,IL-6 levels,endometrial fibrosis area ratio,MMP-2 and MMP-9 mRNA expression levels were significantly higher in the model group,while the TXB2,E2,P levels,endometrial thickness,number of glands,endometrial MVD,and VEGF mRNA expression were significantly lower(P<0.05).Compared with the model group,the amount of uterine bleeding,the plasma 6-keto-PGF1α,TNF-α,IL-6 levels,endometrial fibrosis area ratio,MMP-2 and MMP-9mRNA expression levels were significantly lower in the Panax notoginseng saponins high-dose groups,while the TXB2,E2levels,endometrial thickness,number of glands,endometrial MVD,and VEGF mR

关 键 词：三七皂甙 流产 不全 子宫出血 子宫内膜 炎症 血管重塑 三七总皂苷 

分 类 号：R714.21[医药卫生—妇产科学]