U1 small nuclear ribonucleoprotein is essential for early larval development in silkworm, Bombyx mori  

作　　者：Xiaoqian Zhang Dehong Yang Xu Yang Xinran Zhang Yong Zhang Yongping Huang Shengxiang Zhang Zulian Liu 

机构地区：[1]College Forestry,Shangdong Agriculture University,Shangdong Province,Taian,China [2]Key Laboratory of Insect Developmental and Evolutionary Biology,Center for Excellence in Molecular Plant Sciences,Shanghai Institute of Plant Physiology and Ecology,Chinese Academy of Sciences,Shanghai,China [3]CAS Center for Excellence in Biotic Interactions,University of Chinese Academy of Sciences,Beijing,China [4]Soochow University,Suzhou,Jiangsu Province,China

出　　处：《Insect Science》2022年第2期379-387,共9页昆虫科学（英文版）

基　　金：supported by grants from the National Science Foundation of China(31830093,32021001 and 31802005);Strategic Priority Research Program of Chinese Academy of Sciences(XDB11010600).

摘　　要：U1 small nuclear ribonucleoproteins(U1 snRNP)associates with 5ʹsplice sites in the form of ribonucleoprotein particles and is highly conserved among species.The physiological functions of U1 snRNP in a lepidopteran model insect Bombyx mori is mostly unknown.Here,we showed that U1 snRNP plays an important role in the development of silkworm.Knockout of U1 snRNP in silkworm showed either delayed or stationary 1st instar larva development compared with the wild-type group.U1 snRNP deletion mutants exhibited abnormal cellular phenotypes with enlarged cell nucleus,scant cytoplasm and enlarged nuclei.RNA-seq analysis revealed that genes involved in metabolic pathway,biosynthesis of secondary metabolites and steroid hormone biosynthesis were significantly affected by U1 snRNP depletion.Taken together,our study suggests that U1 snRNP homeostasis plays an important role in silkworm development.

关 键 词：CRISPR/Cas9 development ECDYSONE SILKWORM U1 snRNP 

分 类 号：S881[农业科学—特种经济动物饲养]