A venom protein of ectoparasitoid Pachycrepoideus vindemiae, PvG6PDH, contributes to parasitism by inhibiting host glucose-6-phosphate metabolism  

作　　者：Lei Yang Liming Qiu Qi Fang Shaoying Wu Gongyin Ye 

机构地区：[1]Hainan University,Haikou,570228 China [2]State Key Laboratory of Rice Biology&Ministry of Agricultural and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insects,Institute of Insect Sciences,Zhejiang University,Hangzhou,310058 China

出　　处：《Insect Science》2022年第2期399-410,共12页昆虫科学（英文版）

基　　金：supported by the Hainan Provincial Natural Science Foundation of China(320RC473);the Key Program of National Natural Science Foundation of China(31830074);the Major International(Regional)Joint Research Project of NSFC(31620103915);the Program for Chinese Innovation Team in Key Areas of Science and Technology of the Ministry of Science and Technology of the People's Republic of China(2016RA4008);the Program for Chinese Outstanding Talents in Agricultural Scientific Research of the Min・istry of Agriculture and Rural Affairs of the People's Republic of China;the Hainan University Foundation(KYQD(ZR)1963,KYQD(ZR)1951).

摘　　要：To achieve successful development,female parasitoids,while laying eggs,introduce various virulence factors,mainly venoms,into host insects to manipulate their physiology.Although numerous studies have been conducted to characterize the components of venoms that regulate host immune responses,few systematic investigations have been conducted on the roles of venom proteins in host metabolic regulation.In this investigation,we characterized a novel venom protein in Pachycrepoideus vindemiae called glucose-6-phosphate dehydrogenase(PvG6PDH)and showed it has a vital role in regulating host carbohydrate metabolism.PvG6PDH encodes 510 amino acids and features a signal peptide and two conserved“G6PDH”domains.Multiple sequence alignment showed it has high amino acid identity with G6PDH from other pteromalids,and quantitative polymerase chain reaction analysis and immunofluorescent staining demonstrated a significantly higher expression of PvG6PDH in the venom apparatus compared with the carcass.We report that PvG6PDH contributes to parasitism by inhibiting the glucose-6-phosphate(G6P)metabolism of host Drosophila melanogaster,as demonstrated by PvG6PDH injection and RNA interference analysis.Further tests revealed that the accumulation of host G6P was caused by the transcriptional inhibition of G6P-metabolism-related genes.These findings greatly contribute to our understanding of venom-mediated host metabolic regulation,further laying the foundation for the development of venom proteins as biological agents for pest control.

关 键 词：biological control metabolism Pachycrepoideus vindemiae PARASITOID VENOM 

分 类 号：Q96[生物学—昆虫学]